What is the interpretation of the following lab results: TB1 antigen level of 0.05 International Units per milliliter (IU/mL), TB2 antigen level of 0.03 IU/mL, mitogen level of 0.070 IU/mL, and an indeterminate result for the tuberculosis gamma interferon release assay (TB-GIPA) with a TB glycoprotein (TBGP) test?

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Last updated: April 18, 2025View editorial policy

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From the Guidelines

The patient's QuantiFERON-TB Gold test result is indeterminate, and I recommend repeating the test to obtain a definitive result. The indeterminate result is likely due to the low mitogen control value (0.070 IU/mL), which suggests a possible issue with the patient's immune response or test processing 1. The TB antigen responses (TB1: 0.05 IU/mL, TB2: 0.03 IU/mL) are low, but cannot be interpreted as negative due to the inadequate mitogen response. Some key points to consider in this case include:

  • The patient's immune status, as indeterminate results are more common in people with weakened immune systems 1
  • The need for alternative testing, such as a tuberculin skin test (TST), if the repeat QuantiFERON-TB Gold test is also indeterminate
  • The importance of evaluating the patient for symptoms suggestive of TB (such as persistent cough, weight loss, night sweats) or risk factors for TB exposure, and considering further testing (e.g., chest X-ray, sputum testing) if necessary
  • The potential for TST to cause boosting and the need for two-step testing in settings conducting serial testing, as noted in the guidelines for using the QuantiFERON-TB Gold test 1

From the Research

Interpretation of Test Results

  • The provided test results show the following values:
    • Nil: 0.04 IU/mL
    • TB1 antigen: 0.05 IU/mL
    • TB2 antigen: 0.03 IU/mL
    • Mitogen: 0.070 IU/mL
    • TBGP: INDETERMINATE, negative, abnormal
  • These results are from the Valley Medical Group, dated 04-18-2025

Treatment of Tuberculosis

  • According to the study 2, the preferred treatment for patients with fully susceptible organisms is a 6-month regimen consisting of isoniazid, rifampin, and pyrazinamide, followed by isoniazid and rifampin for 4 months
  • The study 3 states that first-line therapy for active tuberculosis has remained unchanged for nearly 40 years, with isoniazid, rifampin, pyrazinamide, and ethambutol given for the initial two-month phase, followed by isoniazid and rifampin for 4 to 7 months
  • The study 4 recommends that initial therapy for patients with active or suspected tuberculosis should include isoniazid, rifampin, pyrazinamide, and ethambutol until susceptibility is known

Treatment of Latent Tuberculosis

  • The study 3 mentions that rifamycin-based regimens for latent TB infection have been successful in preventing progression to TB disease
  • The study 4 states that the standard treatment for latent tuberculosis is nine months of isoniazid taken daily, or twice weekly under direct observation by a healthcare worker

Pharmacokinetics of Tuberculosis Drugs

  • The study 5 evaluated the pharmacokinetics of rifampin, isoniazid, pyrazinamide, and ethambutol in a cohort of tuberculosis patients and identified several factors associated with variations in antituberculosis drug concentrations, including human immunodeficiency virus infection, formulation factors, and patient characteristics such as age and sex.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Updates in the Treatment of Active and Latent Tuberculosis.

Seminars in respiratory and critical care medicine, 2018

Research

Drugs for tuberculosis.

Treatment guidelines from the Medical Letter, 2012

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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