What thyroid function tests and monitoring are recommended for patients taking Keytruda (pembrolizumab)?

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Thyroid Monitoring for Keytruda (Pembrolizumab)

Baseline and Routine Monitoring Protocol

Patients on Keytruda require TSH and free T4 monitoring before every infusion for the first 3 months, then every second cycle thereafter (for 2-weekly schedules). 1

Initial Assessment

  • Measure TSH and free T4 (FT4) before starting pembrolizumab 1
  • Baseline abnormal thyroid values do not preclude treatment, but discuss with endocrinology if uncertain 1
  • Check TSH 4-6 weeks after cycle 4 (with restaging CT) 1

Ongoing Monitoring Schedule

  • First 3 months: Check TSH (and optionally FT4) every cycle 1
  • After 3 months: Check TSH every second cycle for 2-weekly infusion schedules 1
  • Continue monitoring even if asymptomatic, as thyroid dysfunction occurs in 5-10% of patients on anti-PD-1 therapy like pembrolizumab 1

Incidence and Timing of Thyroid Dysfunction

Thyroid dysfunction with pembrolizumab occurs in approximately 21% of patients, with 80% of affected patients developing anti-thyroid antibodies. 2 The dysfunction typically manifests early, with a median onset of 42 days after starting treatment. 2

Clinical Pattern

  • Hyperthyroidism phase: Often transient, occurring first in 60% of patients who develop thyroid dysfunction 2
  • Hypothyroidism phase: Follows hyperthyroidism or occurs de novo, usually requiring long-term thyroid hormone replacement 1
  • Most thyroid dysfunction is asymptomatic and detected only through routine blood testing 1

Interpretation of Results

Normal TSH with Normal FT4

  • Continue pembrolizumab without interruption 1
  • Maintain scheduled monitoring 1

Elevated TSH (Hypothyroidism)

For TSH >10 mIU/L or any TSH elevation with low FT4:

  • Start levothyroxine 0.5-1.5 μg/kg (start low in elderly or those with cardiac history) 1
  • Continue pembrolizumab—do not interrupt immunotherapy 1
  • Consider propranolol or atenolol for symptomatic relief 1

For TSH elevation with normal FT4 (subclinical hypothyroidism):

  • Consider thyroid hormone replacement even for subclinical hypothyroidism if fatigue or other hypothyroid complaints are present 1
  • Continue pembrolizumab 1

Low TSH with Elevated FT4 (Hyperthyroidism)

This typically represents transient thyroiditis:

  • Start propranolol or atenolol for symptomatic control (tachycardia, tremor, anxiety) 1
  • Consider carbimazole if anti-TSH receptor antibodies are positive 1
  • Continue pembrolizumab if patient is well 1
  • If patient is unwell, withhold pembrolizumab temporarily and restart when symptoms are controlled 1

For painful thyroiditis:

  • Consider prednisolone 0.5 mg/kg with taper 1

Falling TSH Across Two Measurements with Normal or Low T4

This pattern suggests pituitary dysfunction (hypophysitis):

  • Perform weekly cortisol measurements 1
  • Rule out adrenal insufficiency before starting or increasing thyroid hormone, as this can precipitate adrenal crisis 1
  • If hypophysitis is confirmed, start physiologic dose steroids 1 week prior to thyroid hormone replacement 1

Critical Management Principles

When to Continue Pembrolizumab

  • Continue immunotherapy in nearly all cases of thyroid dysfunction 1
  • Thyroid dysfunction rarely requires treatment interruption 1
  • High-dose corticosteroids are rarely required for thyroid-related adverse events 1

When to Temporarily Hold Pembrolizumab

  • Only withhold if patient is significantly unwell from hyperthyroidism 1
  • Restart when symptoms are controlled with beta-blockers or other symptomatic management 1

Hormone Replacement Considerations

  • Thyroid hormone replacement is usually long-lasting and often permanent 1
  • Even subclinical hypothyroidism warrants treatment consideration if fatigue or other complaints are present 1
  • Do not reduce or discontinue pembrolizumab due to thyroid dysfunction—it does not necessitate dose modification 1

Important Caveats

Iodine Exposure

  • CT contrast containing iodine can transiently impact thyroid function tests 1
  • Consider timing of imaging studies when interpreting borderline results 1

Late Endocrine Dysfunction

  • Thyroid dysfunction can occur late in the treatment course 1
  • Maintain vigilance even after completing pembrolizumab therapy 1

Prognostic Significance

  • Development of thyroid dysfunction during pembrolizumab may be associated with improved overall survival (HR 0.29, p=0.04) 2
  • This suggests thyroid dysfunction should not be viewed as a reason to discontinue effective cancer therapy 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Antibody-mediated thyroid dysfunction during T-cell checkpoint blockade in patients with non-small-cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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