When should I take anastrozole (Arimidex) after my testosterone injection for testosterone replacement therapy?

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Last updated: February 7, 2026View editorial policy

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Anastrozole Should Not Be Routinely Used with Testosterone Replacement Therapy

You should not take anastrozole with testosterone replacement therapy (TRT) unless you develop specific clinical problems like gynecomastia or significantly elevated estradiol levels causing symptoms. The evidence provided addresses anastrozole exclusively in the context of breast cancer prevention and treatment in women—not for routine use in men receiving TRT.

Critical Evidence Gap

The guidelines and drug labels provided focus entirely on anastrozole for breast cancer risk reduction in postmenopausal women 1. These sources explicitly state that anastrozole should not be prescribed for breast cancer risk reduction in premenopausal women 1, and provide no guidance whatsoever for its use in men receiving testosterone therapy.

When Anastrozole Timing Matters (If Prescribed Off-Label)

If your physician has prescribed anastrozole off-label alongside TRT, the research evidence suggests:

Dosing Patterns from Research Studies

  • Daily dosing of 1 mg anastrozole has been studied when combined with testosterone pellet insertions 2
  • Men receiving this combination maintained therapeutic testosterone levels significantly longer (198 days vs 128 days) compared to testosterone alone 2
  • Subcutaneous anastrozole (mean 15.3 mg) delivered simultaneously with testosterone implants maintained low estradiol levels throughout a 4.8-month implant cycle 3

Monitoring Timeline After Starting

Based on testosterone monitoring guidelines (which would apply if anastrozole is added):

  • Measure testosterone and estradiol levels at 2-3 months after initiating or changing the regimen 4
  • For injectable testosterone, measure levels midway between injections (day 5-7 for weekly injections) to assess treatment efficacy 4
  • Continue monitoring at 3-6 month intervals for the first year, then yearly 4

Important Safety Considerations

Bone Health Risks

  • Anastrozole causes increased bone loss in postmenopausal women 1
  • Prior to initiating anastrozole, baseline fracture risk assessment and bone mineral density measurement are required 1
  • All patients should receive calcium and vitamin D supplements and engage in regular exercise 1
  • Short-term studies (12 weeks) in elderly hypogonadal men showed no adverse bone effects, possibly due to concomitant testosterone increases 5

Other Side Effects to Monitor

Women taking anastrozole experience: joint stiffness, arthralgias, vasomotor symptoms, hypertension, dry eyes, and vaginal dryness 1. Similar effects may occur in men, though this is not well-studied.

The Bottom Line

There is no established guideline-based indication for routine anastrozole use with TRT. The timing question assumes anastrozole should be taken, but the medical literature does not support this practice as standard care. If prescribed off-label by your physician for specific clinical indications (such as symptomatic gynecomastia or markedly elevated estradiol), it would typically be taken daily (1 mg) starting simultaneously with testosterone therapy 2, 3, with monitoring at 2-3 months 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Subcutaneous Testosterone Anastrozole Therapy in Men: Rationale, Dosing, and Levels on Therapy.

International journal of pharmaceutical compounding, 2019

Guideline

Timing of Testosterone Level Measurements After Testosterone Replacement Therapy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Effect of aromatase inhibition on bone metabolism in elderly hypogonadal men.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2005

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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