What is the mechanism of action of isoprenaline (isoproterenol)?

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Mechanism of Action of Isoproterenol

Isoproterenol is a potent nonselective beta-adrenergic agonist with very low affinity for alpha-adrenergic receptors, producing both chronotropic and inotropic effects on cardiac myocytes while simultaneously causing peripheral vasodilation. 1

Primary Receptor Activity

  • Isoproterenol acts as a nonselective beta agonist, stimulating both β1 and β2 adrenergic receptors throughout the body 2
  • The drug has very low affinity for alpha-adrenergic receptors, distinguishing it from mixed catecholamines like epinephrine 1
  • It is a relatively poor substrate for monoamine oxidase (MAO) and is not taken up by sympathetic neurons to the same extent as epinephrine and norepinephrine 1

Cardiovascular Effects

Cardiac Actions (β1-mediated)

  • Enhances sinus node automaticity and facilitates sinoatrial conduction, increasing heart rate (positive chronotropy) 2
  • Increases myocardial contractility (positive inotropy), enhancing cardiac output 2
  • Enhances atrioventricular nodal function, improving conduction through the AV node 2

Vascular Effects (β2-mediated)

  • Lowers peripheral vascular resistance, primarily in skeletal muscle but also in renal and mesenteric vascular beds 1
  • Causes diastolic blood pressure to fall due to peripheral vasodilation 1
  • Systolic blood pressure may remain unchanged or rise, although mean arterial pressure typically falls 1
  • Produces pulmonary artery vasodilation 3

Metabolic Effects

  • Stimulates the release of free fatty acids and energy production, equally effective as epinephrine in this regard 1
  • Causes less hyperglycemia than epinephrine 1

Smooth Muscle Effects

  • Relaxes bronchial smooth muscle, preventing or relieving bronchoconstriction through β2 receptor stimulation 1, 4
  • Relaxes gastrointestinal smooth muscle when tone is high 1
  • Tolerance to the bronchodilator effect develops with overuse 1

Pharmacokinetic Considerations

  • Elimination half-life is extremely short at 2-5 minutes when administered intravenously, requiring continuous infusion for sustained effect 5
  • Metabolized primarily in the liver and other tissues by catechol-O-methyltransferase (COMT) 1
  • The duration of action may be longer than epinephrine but is still brief 1

Critical Clinical Implications

Oxygen Demand vs. Supply Mismatch

  • Isoproterenol increases myocardial oxygen demand through β1 effects (increased heart rate and contractility) while simultaneously decreasing coronary perfusion through β2-mediated effects 2, 3
  • This creates a dangerous mismatch and is why the drug should be avoided in settings where coronary ischemia is a concern 2

Hemodynamic Profile

  • Cardiac output increases because of positive inotropic and chronotropic effects occurring in the face of diminished peripheral vascular resistance 1
  • The combination of increased cardiac work and decreased afterload makes it useful for bradycardia but problematic in hypotensive states requiring peripheral vasoconstriction 3

Paradoxical Effects

  • In rare cases (approximately 7% of patients), isoproterenol can paradoxically cause bradycardia, either sinus/junctional bradycardia or second-degree AV block, particularly in patients with underlying conduction abnormalities or hypervagotonia 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Isoproterenol's Effects on Afterload and Cardiac Performance

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Isoproterenol Pharmacokinetics and Clinical Use

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Paradoxical effect of isoprenaline infusion.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 2005

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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