What are normal serum β‑human chorionic gonadotropin (β‑hCG) levels at 13 weeks gestation in a singleton pregnancy?

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Normal hCG Levels at 13 Weeks Gestation

At 13 weeks gestation in a singleton pregnancy, serum β-hCG levels are declining from their peak and typically range widely, but specific "normal" reference ranges are not clinically used because hCG levels vary enormously between individuals and ultrasound—not hCG—is the standard for dating and assessing pregnancy viability at this gestational age.

Why hCG Levels Are Not Used for Assessment at 13 Weeks

  • hCG peaks around 8-12 weeks of gestation at approximately 100,000 mIU/mL, then declines steadily through week 16 and beyond 1, 2, 3.

  • Free beta-hCG, intact hCG, and hyperglycosylated hCG all decrease by approximately 20-40% from 11 to 13 completed weeks of gestation, making this a period of rapid decline 1.

  • The American College of Radiology explicitly recommends using ultrasound dating—not hCG levels—to establish accurate gestational age, as hCG levels have poor accuracy for dating beyond 6 weeks 1.

  • At 13 weeks gestational age, crown-rump length measurement by transvaginal ultrasound provides gestational age estimates accurate to within 7 days, far superior to any hCG-based estimate 1.

Clinical Context: When hCG Is Measured at 13 Weeks

  • First-trimester combined screening for Down syndrome uses hCG (or free beta-hCG) at 11-13 weeks, but these are interpreted as multiples of the median (MoM) adjusted for gestational age, maternal weight, and other factors—not as absolute values 4, 1.

  • At 13 weeks, intact hCG may perform slightly better than free beta-hCG for Down syndrome screening, with a 1-2% higher detection rate 4, 1.

  • In Down syndrome pregnancies, hCG levels are typically elevated (higher MoM), while in trisomy 18 pregnancies, hCG levels are typically reduced (lower MoM) 5.

The Wide Variability Problem

  • A single hCG measurement at any gestational age has enormous inter-individual variability; at 6 weeks, viable pregnancies can range from 1,094 mIU/mL to over 25,000 mIU/mL 6.

  • By 13 weeks, after the peak and during the decline phase, absolute values are even less clinically meaningful because the rate of decline varies significantly between individuals 3.

  • Different hCG assays detect different isoforms (free beta-hCG, intact hCG, hyperglycosylated hCG) with varying sensitivities, further complicating interpretation of absolute values 1.

What Should Be Done at 13 Weeks Instead

  • Transvaginal ultrasound at 11-13 weeks should document:

    • Crown-rump length for accurate gestational age dating
    • Presence of cardiac activity
    • Nuchal translucency measurement (if performing first-trimester screening)
    • Number of gestations and chorionicity if multiple 4
  • If first-trimester combined screening is being performed, hCG (or free beta-hCG) is measured alongside PAPP-A and nuchal translucency, achieving detection rates of 82-86% for Down syndrome at a 5% false-positive rate 4, 1.

Critical Pitfall to Avoid

  • Never attempt to use an absolute hCG value at 13 weeks to assess pregnancy viability or normalcy; this is the role of ultrasound visualization of fetal structures and cardiac activity 4, 1.

  • The only clinical utility of hCG at 13 weeks is as part of aneuploidy screening, where it is interpreted as a risk-adjusted multiple of the median—not as a standalone absolute value 4, 5.

References

Guideline

Beta-hCG Levels and Pregnancy Progression

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Maternal plasma beta-hCG in early human pregnancy.

British journal of obstetrics and gynaecology, 1980

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

hCG and Progesterone Testing Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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