What is the recommended first‑line therapy for an adult with confirmed Helicobacter pylori infection, including alternatives for drug allergies and follow‑up testing?

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Last updated: February 22, 2026View editorial policy

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H. Pylori Treatment

First-Line Therapy: Bismuth Quadruple Therapy for 14 Days

Bismuth quadruple therapy for 14 days is the definitive first-line treatment for adults with confirmed H. pylori infection, achieving 80–90% eradication rates even in regions with high clarithromycin and metronidazole resistance. 1, 2, 3

Recommended Regimen Components

  • Esomeprazole or rabeprazole 40 mg twice daily (preferred over other PPIs; increases cure rates by 8–12%) 1, 2, 3
  • Bismuth subsalicylate 262 mg (two tablets) four times daily 1, 2, 3
  • Metronidazole 500 mg three to four times daily (total 1.5–2 g/day) 1, 2, 3
  • Tetracycline 500 mg four times daily 1, 2, 3

Critical Administration Instructions

  • Take PPI 30 minutes before meals on an empty stomach; avoid concomitant antacids 1, 2, 3
  • Take bismuth 30 minutes before meals and at bedtime 2
  • Take metronidazole 30 minutes after meals 2
  • 14-day duration is mandatory—extending from 7 to 14 days improves eradication by approximately 5% 1, 2, 3, 4

Why Bismuth Quadruple Therapy Is First-Line

Clarithromycin resistance now exceeds 15–20% across North America and Europe, reducing traditional triple therapy success to only ~70% 1, 3. When clarithromycin-resistant strains are present, triple therapy eradication drops from ~90% to ~20% 1, 3. In contrast, bismuth quadruple therapy maintains 80–90% efficacy even against dual clarithromycin-metronidazole resistant strains because bismuth's synergistic effect overcomes metronidazole resistance in vitro 5, 1, 3, 6. Additionally, no bacterial resistance to bismuth has been described, and tetracycline resistance remains rare (<5%) 1, 3.

Alternative First-Line Option (When Bismuth Unavailable)

Concomitant non-bismuth quadruple therapy may be used only if bismuth is unavailable AND local clarithromycin resistance is documented <15%. 1, 2, 3

  • Esomeprazole or rabeprazole 40 mg twice daily 1
  • Amoxicillin 1000 mg twice daily 1
  • Clarithromycin 500 mg twice daily 1
  • Metronidazole 500 mg twice daily 1
  • Duration: 14 days 1, 4

Do not use clarithromycin-based triple therapy empirically unless local surveillance confirms resistance <15%—most regions now exceed this threshold 1, 3.

Second-Line Therapy After First-Line Failure

After Bismuth Quadruple Therapy Fails

Levofloxacin triple therapy for 14 days is the recommended second-line option, provided the patient has no prior fluoroquinolone exposure. 5, 1, 2, 3

  • Esomeprazole or rabeprazole 40 mg twice daily 1
  • Amoxicillin 1000 mg twice daily 5, 1
  • Levofloxacin 500 mg once daily (or 250 mg twice daily) 5, 1
  • Duration: 14 days 1

Critical caveat: Levofloxacin resistance is rising globally (primary 11–30%, secondary 19–30%) 1, 3. Never use levofloxacin in patients with chronic bronchopneumopathy or any prior fluoroquinolone exposure for any indication, as cross-resistance is universal 5, 1.

After Clarithromycin-Based Triple Therapy Fails

Switch to bismuth quadruple therapy for 14 days. 1, 2, 3 Never repeat clarithromycin if it was in the failed regimen—resistance develops rapidly after exposure, dropping eradication from ~90% to ~20% 1, 3.

Third-Line and Rescue Therapies

After two documented treatment failures with confirmed patient adherence, antibiotic susceptibility testing should guide further treatment whenever possible. 5, 1, 2, 3

Empiric Third-Line Options (When Susceptibility Testing Unavailable)

Rifabutin triple therapy for 14 days:

  • Rifabutin 150 mg twice daily 1, 2, 3
  • Amoxicillin 1000 mg twice daily 1, 2, 3
  • Esomeprazole or rabeprazole 40 mg twice daily 1, 2, 3

Rifabutin resistance is rare, making this an effective rescue option 1, 3. However, reserve rifabutin for at least third-line due to potential myelotoxicity 4.

High-dose dual therapy for 14 days (alternative rescue):

  • Amoxicillin 2–3 g daily in 3–4 divided doses 1, 2, 3
  • Esomeprazole or rabeprazole 40 mg twice daily 1, 2, 3

This simpler regimen may be useful in elderly or complex patients 2, 3.

Special Populations: Penicillin Allergy

In patients with confirmed penicillin allergy, bismuth quadruple therapy is the first-choice regimen because it contains tetracycline instead of amoxicillin. 5, 1, 2, 3

Consider penicillin allergy testing after first-line failure—most reported allergies are not true, and amoxicillin resistance remains rare (<5%) 1, 2, 3.

If bismuth is unavailable and clarithromycin resistance is <15%:

  • PPI + clarithromycin + metronidazole for 14 days 5

For second-line in penicillin-allergic patients (if no prior fluoroquinolone exposure):

  • PPI + clarithromycin + levofloxacin for 14 days 5

Confirmation of Eradication (Test-of-Cure)

Perform urea breath test or validated monoclonal stool antigen test at least 4 weeks after completing therapy and at least 2 weeks after stopping PPI. 1, 2, 3

Never use serology for test-of-cure—antibodies persist long after successful eradication 1, 3.

Patient Factors That Reduce Success

  • Smoking roughly doubles the odds of treatment failure (OR ≈1.95); advise cessation during therapy 1, 2
  • Elevated BMI/obesity may lower gastric mucosal drug concentrations, potentially reducing efficacy 1, 2
  • Poor compliance is a leading cause of failure; provide clear written instructions and emphasize completing the full 14-day course 1, 2, 3

Expected Side Effects

Diarrhea occurs in 21–41% of patients during the first week due to gut microbiota disruption; this does not indicate treatment failure 1. Consider adjunctive probiotics to reduce diarrhea risk and improve compliance 1.

Critical Pitfalls to Avoid

  • Never use once-daily PPI dosing—it is a major cause of treatment failure 1, 2, 3
  • Avoid pantoprazole (40 mg provides acid suppression equivalent to only ~9 mg omeprazole) 1, 3
  • Do not shorten therapy below 14 days—this reduces eradication by ~5% 1, 2, 3, 4
  • Never repeat clarithromycin or levofloxacin if they were in a failed regimen 1, 2, 3
  • Do not assume low clarithromycin resistance without local surveillance data—most regions now exceed 15–20% 1, 3
  • Do not use levofloxacin empirically as first-line therapy—this accelerates resistance development 1, 3

References

Guideline

Helicobacter Pylori Infection Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

First-Line Bismuth Quadruple Therapy for *Helicobacter pylori* Eradication

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Bismuth Quadruple Therapy as First‑Line Treatment for *Helicobacter pylori* Infection

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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