How do glucocorticoids and nonsteroidal anti‑inflammatory drugs differ in their anti‑inflammatory mechanisms regarding phospholipase A₂ and cyclooxygenase inhibition?

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Last updated: February 22, 2026View editorial policy

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Corrected Statement for USMLE

The original sentence is correct: Glucocorticoids inhibit phospholipase A₂, reducing both prostaglandins and leukotrienes, whereas NSAIDs inhibit cyclooxygenase and reduce prostaglandin production only.

Mechanism of Glucocorticoid Anti-Inflammatory Action

Glucocorticoids act upstream in the arachidonic acid cascade by inhibiting phospholipase A₂ (PLA₂), thereby preventing the release of arachidonic acid from membrane phospholipids. 1, 2 This mechanism explains their superior anti-inflammatory efficacy compared to NSAIDs, as they block the formation of both:

  • Prostaglandins (via the cyclooxygenase pathway) 1, 3
  • Leukotrienes (via the lipoxygenase pathway) 1, 3

Molecular Mechanisms of Glucocorticoid Action

Glucocorticoids work through multiple complementary pathways:

  • Induction of lipocortin synthesis: Glucocorticoids induce a family of phospholipase inhibitory proteins (lipocortins) that inhibit PLA₂, phospholipase C, and phosphatidylinositol phospholipase C 1
  • Direct enzyme suppression: In inflammatory contexts, glucocorticoids inhibit the expression of both PLA₂ and cyclooxygenase-2 (COX-2) at transcriptional and translational levels 2, 4, 5
  • Constitutive COX-1 is unaffected: Under normal physiological conditions, COX-1-mediated prostaglandin synthesis is not affected by glucocorticoids 2

Mechanism of NSAID Anti-Inflammatory Action

NSAIDs act downstream by inhibiting cyclooxygenase enzymes (COX-1 and/or COX-2), thereby blocking only prostaglandin synthesis. 6 They have no effect on leukotriene production because they do not inhibit the lipoxygenase pathway. 3

Types of NSAIDs Based on COX Selectivity

  • Non-selective NSAIDs: Reversibly inhibit both COX-1 and COX-2 (e.g., ibuprofen, naproxen, indomethacin) 6, 7
  • Selective COX-2 inhibitors (coxibs): Preferentially inhibit COX-2 over COX-1 due to higher affinity for COX-2 6, 7
  • Aspirin: Irreversibly blocks both COX-1 and COX-2 enzymes 6, 8

Clinical Implications of These Mechanistic Differences

The broader mechanism of glucocorticoids explains why they are more potent anti-inflammatory agents than NSAIDs, but this comes at the cost of more systemic side effects. 3

Key Clinical Considerations

  • Glucocorticoids should be used at the lowest effective dose due to their effects on lipids, glucose tolerance, blood pressure, and bone density, particularly with long-term high-dose therapy 6
  • NSAIDs carry significant gastrointestinal and cardiovascular risks: All NSAIDs increase risk of GI bleeding and ulceration, with non-selective NSAIDs causing approximately 100,000 hospitalizations annually in the United States 6
  • COX-2 selective inhibitors reduce GI complications by approximately 50% compared to traditional NSAIDs, but increase cardiovascular thrombotic risk 6, 7, 8

Common Pitfall to Avoid

Do not assume that glucocorticoids directly inhibit cyclooxygenase like NSAIDs do—this is incorrect. Glucocorticoids reduce prostaglandin synthesis indirectly by preventing arachidonic acid release through PLA₂ inhibition and by suppressing COX-2 expression in inflammatory states, not by directly blocking COX enzyme activity. 2, 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Cyclooxygenase Isoforms

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) Classification and Usage

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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