In a patient with dengue IgG positive, IgM negative, and NS1 (non‑structural protein 1) antigen negative results, what does this serologic pattern indicate and what management is recommended?

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Dengue IgG Positive, IgM Negative, NS1 Negative: Interpretation and Management

This serologic pattern indicates past dengue infection, not acute disease, and requires no specific dengue treatment. 1, 2

Clinical Interpretation

This combination of results has a clear diagnostic meaning:

  • IgG positive with IgM and NS1 negative definitively indicates prior dengue exposure, not current acute infection. 1, 2 IgG antibodies persist for months to years after the initial infection, serving as markers of previous exposure. 3, 1

  • The negative NS1 rules out acute infection because NS1 antigen is detectable from day 1 through day 10 of illness, with peak sensitivity (75-90%) during days 1-5. 1, 4 If the patient were acutely infected, NS1 would be positive during this window. 3, 1

  • The negative IgM further confirms this is not recent infection because IgM appears 3-5 days after symptom onset and remains detectable for 2-3 months. 3, 1 The absence of IgM indicates the infection occurred beyond this 2-3 month window. 3

Management Recommendations

No dengue-specific treatment is indicated for this patient. 1, 2 However, proceed with the following algorithm:

If the Patient is Currently Symptomatic with Fever:

  • Investigate alternative diagnoses because this pattern excludes acute dengue as the cause of current symptoms. 3, 1 The positive IgG simply reflects past exposure, not active disease. 2, 5

  • Consider testing for other febrile illnesses endemic to the patient's exposure area, including malaria, typhoid, leptospirosis, or other flaviviruses like Zika. 3

If Definitive Confirmation is Needed:

  • Perform plaque reduction neutralization testing (PRNT) if you need to distinguish dengue from other flavivirus infections (Zika, yellow fever, Japanese encephalitis), as IgG antibodies cross-react among flaviviruses. 3, 5

  • PRNT titer ≥10 for dengue with <10 for other flaviviruses confirms past dengue infection specifically. 3, 1, 5 However, PRNT is typically only available through public health laboratories or CDC. 3

If the Patient is Asymptomatic:

  • No further testing or treatment is required. 2, 5 Document the positive IgG as evidence of prior dengue immunity. 2

Critical Pitfalls to Avoid

  • Do not treat this as acute dengue infection. The absence of both NS1 and IgM definitively excludes acute disease. 3, 1 Treating based on IgG alone would be inappropriate. 2

  • Do not assume the timing of past infection from IgG alone, as these antibodies persist indefinitely. 1, 2 The specific timing cannot be determined from serology. 3, 1

  • Be aware of flavivirus cross-reactivity. If the patient has travel history to areas with multiple flaviviruses or has received yellow fever or Japanese encephalitis vaccination, the positive IgG may reflect those exposures rather than dengue. 3, 5 Consider PRNT if this distinction matters clinically. 3, 5

  • Do not repeat dengue serologic testing unless the patient develops new symptoms consistent with acute dengue, in which case test with NS1/NAAT and IgM if within 7 days of new symptom onset. 3, 1

Special Considerations for Pregnant Women

  • The interpretation remains the same—past infection, not acute disease. 2 However, if the patient is symptomatic and pregnant, pursue concurrent Zika virus testing given the overlapping clinical presentation and critical implications for pregnancy. 3, 2

References

Guideline

Dengue Infection Diagnosis and Antibody Development

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Dengue Infection Diagnosis and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Dengue IgG Serology: Interpretation and Diagnostic Approach

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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