What are the per‑millilitre concentrations of the clotting factors and proteins in Octaplex (four‑factor prothrombin complex concentrate)?

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Octaplex Composition: Per-Millilitre Concentrations

Octaplex is a four-factor prothrombin complex concentrate containing therapeutic amounts of coagulation factors II, VII, IX, and X, with dosing standardized to factor IX content at approximately 25 IU/mL after reconstitution.

Core Clotting Factor Content

  • Factor IX serves as the reference standard for all 4-factor PCC products, with dosing expressed in international units (IU) of factor IX, where one IU represents the activity of factor IX in 1 mL of normal plasma 1

  • Octaplex contains proportional amounts of all four vitamin K-dependent factors (II, VII, IX, X), with the concentration of factors II and X approximately equivalent to factor IX content 2, 3

  • Factor VII concentration is present in therapeutic amounts, which distinguishes 4-factor PCCs like Octaplex from 3-factor formulations that contain minimal or no factor VII 4, 2

  • The typical reconstitution yields approximately 20-25 IU/mL of factor IX activity, meaning a 500 IU vial reconstitutes to approximately 20-25 mL total volume 2

Anticoagulant Proteins and Additives

  • Proteins C and S are included in variable amounts as natural anticoagulant proteins to help balance the prothrombotic potential of the concentrate 5, 1

  • Small amounts of heparin are incorporated into the formulation to reduce thrombotic risk during administration 5, 1

  • Some formulations may contain antithrombin as an additional safeguard against thrombosis, though this varies between PCC products 1, 3

Product Characteristics

  • Octaplex is supplied as lyophilized powder that requires reconstitution at room temperature before use, allowing for rapid preparation in emergency situations 5, 2

  • The concentrate provides approximately 25-fold higher concentration of vitamin K-dependent clotting factors per unit volume compared to fresh frozen plasma 5

  • Viral safety is ensured through pathogen reduction and inactivation processes during manufacturing 5

Biochemical Variability

  • Significant differences exist between commercial PCC products in terms of coagulation inhibitor capacity, purity, and exact factor ratios, even among 4-factor formulations 3

  • Batch-to-batch variability may occur within the same brand, which can be clinically relevant for patients requiring frequent infusions 5

  • Octaplex showed no detectable activated coagulation factors in biochemical comparison studies, confirming product safety regarding thrombotic activation 3

Clinical Dosing Context

  • Standard clinical dosing is weight-based using factor IX units per kilogram (e.g., 25-50 IU/kg depending on INR), not per-millilitre concentrations, because the reconstituted volume varies by vial size 5, 1

  • A typical 500 IU vial reconstitutes to approximately 20 mL, yielding roughly 25 IU/mL, though practitioners should always verify the specific product insert for exact reconstitution volumes 2

References

Research

Perioperative use of prothrombin complex concentrates.

Minerva anestesiologica, 2012

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Reversal of Anticoagulation with 4-Factor Prothrombin Complex Concentrate

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

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This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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