GHK-Cu Supply Duration Calculation
A 50 mg vial of GHK-Cu will last 25 days when dosing 2 mg daily for five consecutive days followed by two days off each week.
Mathematical Breakdown
Weekly consumption:
Total duration calculation:
- 50 mg total supply ÷ 10 mg per week = 5 weeks of treatment
- 5 weeks × 5 dosing days per week = 25 total doses
- Calendar time: approximately 35 days (5 weeks)
Dosing Context from Research
The proposed dosing schedule of 2 mg daily appears conservative compared to published research protocols:
- Preclinical intranasal studies used 15 mg/kg daily for two months in aging mice, which improved spatial memory and reduced neuroinflammatory markers 1
- Intra-articular injection studies in rats used concentrations of 0.3-3 mg/mL administered weekly for 4 weeks, showing improved tissue healing 3
- Systemic administration studies for pulmonary fibrosis used 0.2-20 μg/g/day (equivalent to approximately 4-400 mg for a 70 kg human) on alternate days 4
Important Considerations
No established human dosing guidelines exist for GHK-Cu supplementation, as the available evidence comes primarily from preclinical animal studies and topical/wound healing applications 1, 2, 5. The 2 mg daily dose with cycling appears to be an empirical approach without direct clinical validation.
Serum levels decline with age: Natural GHK levels average 200 ng/mL at age 20 but decline to 80 ng/mL by age 60, providing biological rationale for supplementation 2. However, the relationship between exogenous dosing and physiological restoration remains unclear.
Safety profile: GHK-Cu has demonstrated a long history of safe use in wound healing and topical applications, with antioxidant and anti-inflammatory properties 2, 5. However, systemic administration safety data in humans is limited.