Which hepatitis screening tests should be ordered for an adult initiating dialysis for the first time?

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Hepatitis Screening for First-Time Dialysis Patients

For patients initiating in-center hemodialysis, order a comprehensive hepatitis panel consisting of: HBsAg, anti-HBs, anti-HBc for hepatitis B, and anti-HCV antibody (or NAT) for hepatitis C before admission to the dialysis facility. 1, 2

Hepatitis B Screening Requirements

The CDC and Centers for Medicare & Medicaid Services mandate a three-test hepatitis B panel before admission to any outpatient dialysis facility 1, 2:

  • HBsAg (Hepatitis B surface antigen): Identifies active infection (acute or chronic) or carrier state 1
  • Anti-HBs (Antibody to HBsAg): Indicates immunity from vaccination or resolved infection 1
  • Anti-HBc (Antibody to hepatitis B core antigen): Distinguishes natural infection (past or present) from vaccine-induced immunity 1

Why all three tests matter: Testing only HBsAg and anti-HBs misses acute infections during the window period when HBsAg has cleared but anti-HBs hasn't yet developed 1. Testing only HBsAg and anti-HBc fails to identify susceptible patients who need vaccination 1.

Hepatitis C Screening Requirements

The KDIGO 2022 guidelines provide a Grade 1A recommendation to screen all patients upon initiation of in-center hemodialysis using either 1, 2:

  • Anti-HCV antibody immunoassay followed by NAT if positive, OR
  • NAT (nucleic acid testing) alone in high-prevalence dialysis units 1

Critical consideration: In immunocompromised patients or high-prevalence settings, NAT alone as first-line testing avoids false-negatives during the antibody window period 1, 2. Approximately 10% of anti-HCV-negative hemodialysis patients may be HCV RNA-positive due to this window period 1.

Important technical detail: Draw NAT samples before the dialysis session, as hemodialysis reduces viremia levels 1.

Additional Baseline Testing

Establish a baseline ALT level at dialysis initiation for ongoing HCV surveillance, as newly elevated ALT has 83% sensitivity and 90% specificity for acute HCV infection in hemodialysis patients 1.

Common Pitfalls to Avoid

  • Don't skip anti-HBc testing: Economic constraints have led some facilities to screen only HBsAg, but this misses occult HBV infection and fails to distinguish vaccine immunity from natural immunity 3
  • Don't assume resolved HCV provides immunity: Patients with resolved HCV remain at risk for reinfection and require ongoing NAT surveillance every 6 months 2, 4
  • Don't delay testing until after dialysis initiation: All screening must be completed before admission to the outpatient dialysis facility to implement appropriate infection control measures immediately 1, 2

Post-Admission Surveillance Requirements

Once baseline screening is complete, implement ongoing surveillance 1, 2:

  • HCV testing: Every 6 months for all in-center hemodialysis patients (Grade 1B recommendation) 1, 2, 4
  • HBsAg testing: Monthly for susceptible patients (HBsAg-negative, anti-HBs-negative) 1, 2
  • Anti-HBs levels: Annually for immune patients, with revaccination if levels fall below 10 IU/L 1, 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Hepatitis Screening and Surveillance in Dialysis Units

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Hepatitis B status in hemodialysis patients.

Arquivos de gastroenterologia, 2017

Guideline

HCV Control in Dialysis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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