Does Keflex (cephalexin) provide coverage against Gram‑negative rods?

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Last updated: February 27, 2026View editorial policy

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Does Keflex (Cephalexin) Cover Gram-Negative Rods?

Cephalexin provides limited and unreliable coverage against gram-negative rods and should not be relied upon as primary therapy for infections caused by these organisms.

Spectrum of Activity Against Gram-Negative Organisms

Organisms with Documented Activity

  • Cephalexin demonstrates in vitro activity against Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, which are the primary gram-negative rods it can reliably cover 1.
  • The FDA label confirms cephalexin is active against these three Enterobacteriaceae species both in vitro and in clinical infections 1.

Organisms with Poor or No Activity

  • Cephalexin has no activity against Pseudomonas species or Acinetobacter calcoaceticus, making it completely inappropriate for infections involving these pathogens 1.
  • It is not active against most strains of Enterobacter species, Morganella morganii, and Proteus vulgaris 1.
  • Cephalexin demonstrates poor activity against Haemophilus influenzae and Moraxella catarrhalis, limiting its utility in respiratory tract infections where these organisms are common 2.

Clinical Implications and Limitations

When Gram-Negative Coverage Is Inadequate

  • For acute bacterial sinusitis and many respiratory infections, cephalexin should be avoided due to inadequate H. influenzae coverage 2.
  • Cephalexin has inadequate activity against beta-lactamase-producing M. catarrhalis, further restricting its use in upper respiratory infections 2.

Comparative Potency

  • Among first-generation cephalosporins tested against gram-negative bacilli (E. coli, K. pneumoniae, P. mirabilis), cefazolin demonstrates superior activity compared to cephalexin 3.
  • Historical data show that only 49.1% of tested organisms were suppressed by cephalexin at concentrations of 3.13 mcg/mL or less, compared to higher suppression rates with other cephalosporins 3.

Appropriate Clinical Use

  • Cephalexin remains highly effective for gram-positive infections (streptococcal and staphylococcal skin and soft-tissue infections), where it achieves cure rates of 90% or higher 4.
  • For mixed infections or when gram-negative coverage is essential, amoxicillin-clavulanate or broader-spectrum agents should be selected instead 5.

Key Pitfalls to Avoid

  • Do not use cephalexin for polymicrobial infections involving anaerobes or resistant gram-negative organisms, as it lacks adequate coverage 2.
  • Do not prescribe cephalexin for diabetic foot infections or other polymicrobial soft-tissue infections where broader gram-negative and anaerobic coverage is required 5.
  • Avoid cephalexin for bite-related cellulitis (human or animal), where Pasteurella multocida, Eikenella corrodens, and anaerobes require amoxicillin-clavulanate or alternative regimens 5, 6.

References

Guideline

Cephalexin Dosing Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Evaluation of antibacterial activities of cephalosporin antibiotics: cefazolin, cephaloridine, cephalothin, and cephalexin.

Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology, 1975

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Management of Cellulitis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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