What is the recommended loading dose of Polymyxin B for an adult with a serious Gram‑negative infection?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: February 28, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Polymyxin B Loading Dose for Serious Gram-Negative Infections

Administer a loading dose of 2–2.5 mg/kg intravenously on day 1, regardless of renal function, to rapidly achieve therapeutic plasma concentrations. 1

Loading Dose Protocol

  • Give 2–2.5 mg/kg IV as a single loading dose (approximately 20,000–25,000 units/kg, or 1.4–1.75 million international units for a 70-kg adult) on the first day of therapy 1
  • The loading dose is mandatory for all patients, including those with severe renal dysfunction or on continuous renal replacement therapy (CRRT), because renal impairment does not affect initial drug exposure 1
  • This loading dose rapidly achieves the target steady-state average plasma concentration of approximately 3.35 mg/L on day 1, which would otherwise take 2–3 days to reach with maintenance dosing alone 1, 2

Maintenance Dosing Following the Load

  • After the loading dose, initiate maintenance therapy at 1.5–3 mg/kg/day divided into two equal doses every 12 hours (for a 70-kg patient, this equals 105–210 mg/day or 1.05–2.1 million international units/day) 1
  • The standard maintenance regimen most commonly used is 2.5–3.0 mg/kg/day divided into two daily IV doses 3, 4
  • No dose reduction is required for renal impairment, including patients on CRRT—this is the most critical distinction from colistin and contradicts older FDA labeling 1, 5, 6

Critical Renal Function Considerations

  • Polymyxin B clearance is weight-based and not renally dependent, so maintain standard dosing even in severe renal dysfunction 1
  • Studies demonstrate comparable polymyxin B exposures (AUC 63.5 ± 16.6 mg·h/L vs 56.0 ± 17.5 mg·h/L, P = 0.42) in patients with normal renal function versus renal insufficiency receiving the same doses 6
  • Polymyxin B has significantly lower nephrotoxicity than colistin (11.8% vs 39.3%), making it the preferred polymyxin in patients with or at risk for renal impairment 5, 4

Combination Therapy Requirements

  • Always use polymyxin B in combination therapy rather than monotherapy for carbapenem-resistant infections 3, 1, 4
  • For carbapenem-resistant Enterobacterales bloodstream infections, combine with tigecycline or extended-infusion meropenem (1 g over 3 hours every 8 hours) when the meropenem MIC is ≤8 mg/L for CRE or ≤32 mg/L for CRAB 3, 1
  • For ventilator-associated pneumonia caused by carbapenem-resistant pathogens, add adjunctive inhaled colistin (not inhaled polymyxin B) to intravenous polymyxin B 1

Therapeutic Drug Monitoring

  • Target a steady-state average concentration of approximately 3.35 mg/L and an AUC₀₋₂₄h of 50–100 mg·h/L to balance efficacy and minimize toxicity 1, 2
  • Therapeutic drug monitoring is strongly encouraged to individualize dosing and optimize outcomes 3, 1
  • Monitor renal function closely during therapy, as nephrotoxicity remains dose-dependent despite lower rates than colistin 3, 5

Common Pitfalls to Avoid

  • Do not omit the loading dose—failure to administer it results in subtherapeutic levels for the first 24–48 hours, compromising early bacterial killing 1, 4
  • Do not reduce doses in renal impairment—this outdated practice leads to treatment failure, as polymyxin B pharmacokinetics are not significantly affected by creatinine clearance 1, 5, 6
  • Do not confuse polymyxin B with colistin dosing—polymyxin B: 1 mg = 10,000 units; colistin: 1 million IU = 80 mg CMS = 33 mg colistin base activity 3, 1
  • Avoid concurrent nephrotoxic agents (aminoglycosides, NSAIDs, diuretics, ACE inhibitors/ARBs) to minimize additive nephrotoxicity risk 3, 4

Duration of Therapy

  • Hospital-acquired or ventilator-associated pneumonia: 7 days 1
  • Carbapenem-resistant Enterobacterales bloodstream infections: 7–14 days 1
  • Complicated urinary tract infections: 5–7 days 1

Setting and Expertise Requirements

  • Restrict polymyxin B use to healthcare settings with high prevalence of multidrug-resistant Gram-negative pathogens and where clinicians possess specific expertise in its dosing, monitoring, and safety management 3, 1

References

Guideline

Polymyxin B Administration Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Polymyxin B Dosing and Administration in Critical Care

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Polymyxin B in Renal Impairment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.