What screening laboratory tests are indicated for diagnosing peripheral neuropathy?

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Last updated: April 28, 2025View editorial policy

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From the Guidelines

For peripheral neuropathy screening, I recommend a comprehensive approach that includes a careful history, assessment of temperature or pinprick sensation, and vibration sensation using a 128-Hz tuning fork, as well as annual 10-g monofilament testing to identify feet at risk for ulceration and amputation 1. When it comes to lab tests, the focus should be on identifying underlying causes of peripheral neuropathy, with a particular emphasis on diabetes, given its high prevalence as a cause of peripheral neuropathy.

  • A hemoglobin A1c test is crucial for assessing glycemic control in diabetic patients, as it can help prevent diabetic peripheral neuropathy (DPN) and cardiac autonomic neuropathy (CAN) in type 1 diabetes and may modestly slow their progression in type 2 diabetes 1.
  • Additional tests such as vitamin B12 level, folate level, thyroid-stimulating hormone (TSH), and complete blood count (CBC) can help identify other potential causes of peripheral neuropathy, including vitamin deficiencies and thyroid dysfunction.
  • It's also important to consider the patient's overall clinical presentation and risk factors when deciding which tests to order, as some cases may require more specialized testing. The goal of these screenings is not only to diagnose peripheral neuropathy but also to identify and manage underlying conditions that can improve symptoms, reduce sequelae, and enhance quality of life, as the early recognition and appropriate management of neuropathy in people with diabetes is critical 1.

From the Research

Screening Lab Tests for Peripheral Neuropathy

The following lab tests are recommended for screening peripheral neuropathy:

  • Complete blood count 2, 3, 4
  • Comprehensive metabolic profile 2, 3, 4
  • Fasting blood glucose 2, 3, 4
  • Vitamin B12 levels 2, 3, 4
  • Thyroid-stimulating hormone levels 2, 3, 4
  • Serum protein electrophoresis with immunofixation 3
  • Erythrocyte sedimentation rate 2
  • HbA1c 4
  • Renal and liver function tests 4

Specialized Tests

Specialized tests may be ordered if clinically indicated, such as:

  • Electrodiagnostic studies, including nerve conduction studies and electromyography 2, 3
  • Nerve biopsy 2
  • Lumbar puncture and cerebrospinal fluid analysis for diagnosis of Guillain-Barré syndrome and chronic inflammatory demyelinating neuropathy 2
  • Specific antibody assays 3

Note that study 5 is not relevant to the question of screening lab tests for peripheral neuropathy.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Laboratory Tests for Neuropathies: What to do and to Avoid.

Journal of neuromuscular diseases, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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