What is the workup for a missed menstrual period (amenorrhea)?

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From the Guidelines

A missed period workup should begin with a pregnancy test, as pregnancy is the most common cause of a missed period. If negative, further evaluation includes blood tests for hormonal imbalances, including thyroid function tests (TSH, T3, T4), prolactin levels, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol, as suggested by 1. These can identify conditions like thyroid disorders, hyperprolactinemia, or premature ovarian insufficiency. A complete blood count should be performed to rule out anemia.

Initial Evaluation

  • Pregnancy test
  • Blood tests: TSH, T3, T4, prolactin, FSH, LH, estradiol
  • Complete blood count

Additional Tests

  • Pelvic ultrasound to detect structural abnormalities like polycystic ovary syndrome (PCOS) or uterine fibroids, as recommended by 1
  • Testosterone and DHEAS levels for PCOS diagnosis
  • Progesterone challenge (medroxyprogesterone acetate 10mg daily for 5-10 days) in cases of prolonged amenorrhea to determine if estrogen levels are adequate

Lifestyle Modifications

  • If stress, excessive exercise, or significant weight changes are suspected causes, lifestyle modifications should be recommended, as suggested by 1

Importance of Comprehensive Approach

This comprehensive approach helps identify the underlying cause of missed periods, which is essential for appropriate treatment and to prevent potential long-term health consequences like bone density loss or endometrial hyperplasia, as highlighted by 1 and 1.

From the FDA Drug Label

The FDA drug label does not answer the question.

From the Research

Missed Period Workup

The workup for a missed period involves several steps to determine the underlying cause.

  • The most common cause of a missed period is pregnancy, which must be ruled out before proceeding with diagnostic evaluation 2, 3.
  • A careful history and physical examination may reveal evidence of androgen excess, estrogen deficiency, or other endocrinopathies 2.
  • Serum prolactin and thyroid-stimulating hormone (TSH) levels should be checked in all women who are not pregnant 2.
  • If serum prolactin and TSH levels are normal, a progesterone challenge test should be performed to determine outflow tract patency and estrogen status 2.

Diagnostic Tests

  • Galactorrhea by history or on examination and/or an elevated prolactin level should be investigated with an imaging study to rule out a pituitary adenoma 2.
  • In women with hypoestrogenic amenorrhea, indicated by a negative challenge test and a competent outflow tract, serum gonadotropin, follicle-stimulating hormone, and luteinizing hormone levels may be measured to determine whether amenorrhea represents ovarian failure or pituitary or hypothalamic dysfunction 2.
  • Thyroid function tests, including TSH, Thyroxine (T4), Triiodothyronine (T3), Iodine, and Thyroid Autoantibodies, should be assessed to evaluate thyroid function 4, 5.

Special Considerations

  • Hypothalamic amenorrhea is common in women with a history of weight loss, stress, or vigorous exercise 2.
  • Amenorrheic women with adequate estrogen levels should receive cyclic progesterone, and hormonal therapy and calcium supplementation may be necessary in hypoestrogenic amenorrhea 2.
  • Thyroid disorders, particularly subclinical hypothyroidism, can affect fertility and pregnancy outcomes, and routine thyroid function testing is recommended by some guidelines 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Evaluation of amenorrhea.

American family physician, 1996

Research

The missed menstrual period.

Postgraduate medicine, 1977

Research

Thyroid function testing prior to fertility treatment: will we ever agree?

Human reproduction (Oxford, England), 2025

Research

Thyroid function tests during first-trimester of pregnancy: a review of literature.

Pakistan journal of biological sciences : PJBS, 2010

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This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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