What are the diagnostic tests for steatosis (fatty liver disease)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: April 29, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Fatty liver disease can be assessed through a combination of blood tests, imaging studies, and sometimes liver biopsy, with a stepwise approach using blood-based scores and imaging techniques being suitable to rule-out/in advanced fibrosis, as recommended by the most recent guidelines 1.

Initial Evaluation

Initial evaluation typically includes liver function tests such as ALT, AST, and GGT, which may be elevated in fatty liver disease. Complete metabolic panels and lipid profiles are also important to assess for metabolic risk factors.

Imaging Studies

Imaging studies like ultrasound, which can detect moderate to severe fatty infiltration, are often the first-line diagnostic tool due to their non-invasive nature and accessibility.

  • CT scans and MRI, particularly MRI-PDFF (proton density fat fraction) and MR elastography, offer more precise quantification of liver fat and assessment of fibrosis.
  • FibroScan (transient elastography) is another non-invasive option that measures both liver stiffness and fat content.

Definitive Diagnosis

For definitive diagnosis, especially to distinguish between simple steatosis and steatohepatitis or to stage fibrosis, liver biopsy remains the gold standard, though it's typically reserved for cases where the diagnosis remains unclear or when advanced disease is suspected, as noted in earlier guidelines 1.

Importance of Early Detection

These tests are essential because fatty liver disease is often asymptomatic until advanced stages, yet early detection allows for lifestyle interventions and treatment that can prevent progression to cirrhosis or liver failure. The most recent guidelines emphasize the importance of case-finding strategies for MASLD with liver fibrosis, using non-invasive tests, in individuals with cardiometabolic risk factors, abnormal liver enzymes, and/or radiological signs of hepatic steatosis 1.

From the Research

Tests to Assess Fatty Liver

  • Non-invasive assessment of fatty liver is crucial due to the dangers and complications of liver biopsy 2
  • Imaging modalities such as ultrasound, computed tomography (CT), and magnetic resonance imaging (MRI) can be used to assess liver steatosis and detect focal liver lesions (FLLs) 3
  • MRI is generally superior to ultrasound and CT for detection and characterization of FLLs in patients with fatty liver 3
  • Non-invasive diagnostic tests for non-alcoholic fatty liver disease (NAFLD) include ultrasonography (US), CT, and MRI, which can reliably diagnose hepatic steatosis 4
  • Chemical shift-encoded (CSE)-MRI is the best imaging indicator for early liver fat detection 4
  • Clinical prediction rules and blood-based biomarkers, such as NAFLD fibrosis score, FIB-4 index, and BARD score, can be used to identify patients at high risk of NASH and advanced fibrosis 5
  • Combining blood biomarkers can improve diagnostic accuracy, and more expensive tests such as FibroTest or ELF are more specific for detection of patients with significant and advanced fibrosis 5

Non-Invasive Imaging Methods

  • US is widely available and radiation-free but cannot examine the entire liver 4
  • CT is readily available and helpful for detection and risk classification, but exposes users to radiation 4
  • MRI can measure liver fat percentage with magnetic resonance imaging proton density fat fraction (MRI-PDFF) and is expensive and time-consuming 4

Blood-Based Biomarkers

  • NAFLD fibrosis score and FIB-4 index are cost-effective and highly sensitive tools to exclude patients with advanced fibrosis 5
  • FibroTest or ELF are more specific for detection of patients with significant and advanced fibrosis 5
  • Recent efforts have concentrated on "omics" approaches for developing and validating novel biomarkers 5

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.