What is the next step in the workup of a patient with microscopic hematuria (presence of blood in urine) but negative Red Blood Cell (RBC) count on urinalysis microscopy?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: May 5, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

The next step in the workup of a patient with 1+ blood on urinalysis but negative RBCs on microscopy is to repeat the urinalysis to confirm the presence of hematuria, as a single positive dipstick reading without microscopic confirmation does not define microhematuria 1. This approach is based on the American Urological Association (AUA) guidelines, which emphasize the importance of microscopic examination to confirm the diagnosis of microhematuria. According to the AUA guidelines, a positive dipstick test should be followed by microscopic examination to confirm or refute the diagnosis of microhematuria, and if the microscopic examination is negative, the patient should have three additional repeat tests to confirm the presence of hematuria 1. If at least one of the repeat tests is positive on microscopy, then a work-up should be undertaken, which may include cystoscopy and upper tract imaging, depending on the patient's risk factors and symptoms 1. It is also important to consider other potential causes of a positive dipstick test, such as myoglobinuria or hemoglobinuria, and to evaluate for these conditions if suspected. However, based on the most recent and highest quality evidence, repeating the urinalysis to confirm the presence of hematuria is the most appropriate next step in the workup of a patient with 1+ blood on urinalysis but negative RBCs on microscopy. Some key points to consider in the workup of microhematuria include:

  • The definition of microhematuria as three or more red blood cells per high power field on microscopic evaluation of a single specimen 1
  • The importance of considering patient risk factors, such as age, sex, and smoking history, in determining the need for further evaluation 1
  • The role of cystoscopy and upper tract imaging in the evaluation of microhematuria, particularly in patients with risk factors or symptoms suggestive of malignancy 1.

From the Research

Next Steps in Workup

The presence of 1+ blood on urinalysis but negative RBC on microscopy requires further evaluation to determine the cause of the hematuria.

  • The initial step is to repeat the urinalysis to rule out a false-positive result 2.
  • If the result is still positive, additional tests such as a complete blood count, blood urea nitrogen, and creatinine levels may be ordered to assess kidney function 2.
  • Imaging studies like ultrasound or CT scan may also be considered to evaluate the kidneys and urinary tract for any abnormalities 2.
  • It is essential to consider the patient's medical history, physical examination, and other elements of the urinalysis to differentiate between glomerular, renal, and urologic causes of microhematuria 2.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Urinalysis: a comprehensive review.

American family physician, 2005

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.