What is the interpretation of a hepatitis B (hep B) core antibody positive, hepatitis B (hep B) surface antibody and hepatitis B (hep B) surface antigen nonreactive result?

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Last updated: May 11, 2025View editorial policy

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From the Guidelines

This pattern of hepatitis B serology, with a positive core antibody and negative surface antibody and surface antigen, indicates a past hepatitis B infection with resolution but without development of protective immunity. According to the most recent and highest quality study 1, this serologic pattern could represent several scenarios: a resolved infection where surface antibodies have waned over time, a "window period" of acute infection (though unlikely without symptoms or elevated liver enzymes), or a false positive core antibody test. In rare cases, it might indicate occult hepatitis B infection with low-level viral replication.

Key Considerations

  • No immediate treatment is needed, but the patient should be monitored with repeat testing in 3-6 months to confirm this interpretation.
  • Additional testing that may be helpful includes hepatitis B viral DNA to rule out occult infection, and IgM anti-HBc to distinguish between recent and remote infection.
  • Patients with this serologic pattern should be counseled about potential viral reactivation if they become immunosuppressed in the future, as noted in 1 and 1.
  • They should also be screened for other viral hepatitis infections like hepatitis C, as co-infection is common, as mentioned in 1 and 1.

Management and Prevention

  • The risk of HBV reactivation for patients who are HBsAg negative, HBcAb positive varies widely based on the virological profile, disease, and immunosuppressive regimen, as discussed in 1 and 1.
  • Serum HBV DNA testing before the start of therapy may help define their risk of reactivation, as recommended in 1.
  • If viremic, they may receive similar prophylaxis as patients who are HBsAg positive, as suggested in 1.

From the Research

Interpretation of Hepatitis B Test Results

  • A hepatitis B core antibody (anti-HBc) positive result indicates that the individual has been infected with hepatitis B virus (HBV) at some point in their life 2.
  • The presence of hepatitis B surface antibody (anti-HBs) and the absence of hepatitis B surface antigen (HBsAg) suggest that the individual has cleared the virus and is no longer infectious 3, 2.
  • The anti-HBs antibody is recognized as a protective immune marker, indicating that the individual has developed immunity to HBV either through infection and clearance or through vaccination 3.
  • The combination of anti-HBc positivity and anti-HBs positivity, with HBsAg nonreactive, may indicate a past infection that has been cleared, and the individual is no longer at risk of transmitting the virus 2, 4.

Clinical Implications

  • The detection of anti-HBc and anti-HBs antibodies, in the absence of HBsAg, is important for the clinical classification and epidemiological surveillance of HBV infection 2.
  • The level of anti-HBc antibody may be used as a predictor of HBsAg clearance in patients with chronic hepatitis B, particularly those receiving pegylated interferon-alfa therapy 4.
  • A lower level of anti-HBc antibody at week 12 of treatment may indicate a higher likelihood of HBsAg clearance and seroconversion 4.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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