What is the interpretation of the DUTCH (Dried Urine Test for Comprehensive Hormones) test results showing Estradiol (E2) levels, Estrone (E1) levels, alpha-Pregnanediol levels, beta-Pregnanediol levels, beta-Pregnanediol to Estradiol ratio, and Creatinine levels?

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Last updated: May 13, 2025View editorial policy

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From the Research

Based on the DUTCH test results, ovulation appears to have occurred around day 15-16, with a clear estrogen peak followed by a progesterone rise in the luteal phase, as supported by the most recent study 1. The estradiol levels show a classic pattern, rising from follicular phase levels (1.31-2.37 ng/mg) to peak at ovulation (6.23 ng/mg on day 16), then gradually declining through the luteal phase.

  • Key findings include:
    • Estradiol levels peaking at 6.23 ng/mg on day 16, indicating ovulation
    • Progesterone metabolites (a-Pregnanediol and b-Pregnanediol) surging after ovulation, peaking on day 26
    • The b-Pregnanediol/E2 ratio following the expected pattern, being lower during ovulation (<100) and higher during the luteal phase (188-383)
    • Creatinine values remaining within the normal range throughout, suggesting reliable sample collection Progesterone metabolites (a-Pregnanediol and b-Pregnanediol) appropriately surge after ovulation, peaking on day 26, confirming a healthy luteal phase.
  • The study 1 highlights the importance of accurate hormone measurement in determining the timing of ovulation and the fertile window. All hormone levels return to follicular phase ranges by day 34, indicating the cycle is completing normally.
  • The findings of this study 1 are consistent with previous research 2, which demonstrated the reliability of urinary and serum reproductive hormone levels in monitoring the menstrual cycle. This appears to be a healthy hormonal cycle with proper estrogen dominance in the follicular phase transitioning to progesterone dominance after ovulation, with no obvious hormonal imbalances detected.
  • It is essential to consider the most recent and highest-quality evidence when interpreting hormone test results, as emphasized by the study 1.

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This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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