What blood test is used for ovarian cancer screening?

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Last updated: May 13, 2025View editorial policy

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From the Guidelines

There is no reliable blood test for routine ovarian cancer screening in the general population. The CA-125 blood test, which measures a protein that can be elevated in ovarian cancer, is not recommended for general screening because it can be elevated in many non-cancerous conditions (like endometriosis, fibroids, or menstruation) and may not be elevated in early-stage ovarian cancer 1.

Key Points to Consider

  • For high-risk women, such as those with BRCA1/BRCA2 gene mutations or strong family histories of ovarian cancer, doctors may use CA-125 testing along with transvaginal ultrasound for surveillance, but even this combination has limitations in detecting early disease 1.
  • Research is ongoing for more effective screening methods, including panels of multiple biomarkers and more sensitive tests.
  • If you're concerned about ovarian cancer risk, the best approach is to discuss your personal and family history with your doctor, who can assess your risk factors and recommend appropriate monitoring strategies.
  • Be aware of symptoms like persistent bloating, pelvic pain, difficulty eating, and frequent urination, as these warrant prompt medical evaluation.

Limitations of Current Screening Methods

  • The PLCO Cancer Screening Trial found that screening average-risk, asymptomatic women with serum CA-125 testing and transvaginal ultrasonography does not reduce ovarian cancer deaths 1.
  • Harms associated with screening for ovarian cancer have been reported, including false-positive results and unnecessary surgeries 1.

Future Directions

  • Ongoing research aims to develop more effective screening methods, including the use of multiple biomarkers and more sensitive tests.
  • The UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) is assessing multimodality screening with ultrasound and CA-125 versus either ultrasound alone or no screening 1.

From the Research

Blood Test for Ovarian Cancer Screen

  • The primary ovarian cancer marker used for the past four decades is the tumour biomarker CA125 2.
  • Screening average-risk asymptomatic women with CA125 is not recommended by any professional society due to the low sensitivity and specificity of the test in detecting early-stage ovarian cancer 2, 3, 4.
  • The combination of CA125 and transvaginal ultrasound (TVU) has been shown to have a higher sensitivity and specificity than CA125 alone, but still has limitations in detecting early-stage ovarian cancer 3, 5, 6.
  • A study found that the sensitivity and specificity of CA125 and TVU were 40% and 99%, respectively, with a positive predictive value (PPV) of 40% and a negative predictive value (NPV) of 99% 3.
  • Another study identified high-risk categories for predicting the risk of ovarian cancer in women with abnormal CA125 and TVU results, with a sensitivity of 60-85% and specificity of 95-96% 6.
  • The use of point-of-care CA125 assays in primary care facilities may provide a new and valuable target for screening, particularly for low-volume type II ovarian cancer in its early stages of evolution 2.
  • Prophylactic surgery to remove the fallopian tubes and ovaries is recommended for high-risk women, such as those with BRCA1 or BRCA2 mutations, to prevent 90% of ovarian cancers 4, 5.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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