What is the workup for amenorrhea (absence of menstruation)?

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From the Guidelines

Amenorrhea does not require any medical treatment, and the initial step should be to provide reassurance and consider ruling out pregnancy if clinically indicated. The workup for amenorrhea should be guided by the clinical presentation and medical history of the patient. According to 1, if a woman’s regular bleeding pattern changes abruptly to amenorrhea, pregnancy should be ruled out if clinically indicated.

Initial Evaluation

The initial evaluation of amenorrhea should include:

  • A thorough medical history to identify any potential causes of amenorrhea
  • A physical examination to assess for signs of androgen excess, thyroid abnormalities, and galactorrhea
  • Laboratory tests, such as pregnancy test, FSH, LH, prolactin, TSH, and estradiol levels, as indicated by the clinical presentation 1

Further Testing

Further testing may be indicated based on the results of the initial evaluation, and may include:

  • Assessment of androgens (DHEAS, testosterone) if hirsutism is present
  • Pelvic ultrasound to evaluate for polycystic ovary syndrome or structural abnormalities
  • A progesterone challenge test to determine estrogen status
  • Brain MRI if prolactin is elevated to rule out pituitary adenoma 1

Treatment

Treatment of amenorrhea should be individualized and based on the underlying cause, ranging from lifestyle modifications for stress or weight-related amenorrhea to hormone replacement for hypogonadism or specific treatments for conditions like PCOS or hyperprolactinemia. According to 1, if amenorrhea persists and the woman finds it unacceptable, counseling on alternative contraceptive methods and offering another method if desired may be appropriate.

From the Research

Amenorrhea Workup

The workup for amenorrhea typically involves a combination of medical history, physical examination, and laboratory tests.

  • A thorough history and physical examination can help narrow the differential diagnosis of amenorrhea 2, 3, 4, 5, 6.
  • Initial workup of primary and secondary amenorrhea includes a pregnancy test and serum levels of luteinizing hormone, follicle-stimulating hormone, prolactin, and thyroid-stimulating hormone 2, 4, 6.

Primary Amenorrhea

Primary amenorrhea is defined as the absence of menarche by age 15 years 5 or the failure to reach menarche 2.

  • The evaluation of primary amenorrhea includes a thorough health history, physical exam, lab evaluation, and, in some cases, imaging 5.
  • Primary amenorrhea has three broad causes: genetic, hormonal, and anatomic 5.

Secondary Amenorrhea

Secondary amenorrhea is defined as the cessation of regular menses for three months or the cessation of irregular menses for six months 2 or previously regular menses for three months or previously irregular menses for six months 6.

  • Pregnancy should be ruled out in all cases of secondary amenorrhea 2, 3, 4, 6.
  • The treatment of secondary amenorrhea is based on the causative factor and may include hormone replacement therapy, contraception, or infertility services 2, 6.

Laboratory Tests

Laboratory tests for amenorrhea may include:

  • Serum prolactin and thyroid-stimulating hormone (TSH) levels 4
  • Progesterone challenge test to determine outflow tract patency and estrogen status 4
  • Serum gonadotropin, follicle-stimulating hormone, and luteinizing hormone levels to determine whether amenorrhea represents ovarian failure or pituitary or hypothalamic dysfunction 4
  • Karyotyping, serum androgen evaluation, and pelvic or brain imaging may be individualized 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Amenorrhea: an approach to diagnosis and management.

American family physician, 2013

Research

Amenorrhea: evaluation and treatment.

American family physician, 2006

Research

Evaluation of amenorrhea.

American family physician, 1996

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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