What medications can induce a positive Antinuclear Antibody (ANA) test?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: June 1, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Medications such as hydralazine, procainamide, and tumor necrosis factor (TNF) inhibitors like etanercept can induce a positive Antinuclear Antibody (ANA) test. The development of a positive ANA test can occur in patients taking certain medications, including anti-TNF agents, as reported in a study published in the Journal of the American Academy of Dermatology 1. Some of the key medications that can cause a positive ANA test include:

  • Hydralazine
  • Procainamide
  • Isoniazid
  • Minocycline
  • Certain anticonvulsants like phenytoin and carbamazepine
  • Chlorpromazine
  • Methyldopa
  • Quinidine
  • TNF inhibitors such as infliximab and etanercept
  • Biologics, including anti-TNF agents and interferon therapy
  • Certain antibiotics like sulfasalazine and tetracyclines These medications can induce autoantibody production by altering self-antigens, promoting immune complex formation, or directly activating lymphocytes. When evaluating a positive ANA test, it's essential to obtain a thorough medication history to identify potential drug-induced causes before pursuing extensive autoimmune workups, as drug-induced ANA positivity generally doesn't require immunosuppressive treatment beyond stopping the causative medication, as noted in the context of anti-TNF therapy 1.

From the FDA Drug Label

Laboratory Tests Complete blood counts and antinuclear antibody titer determinations are indicated before and periodically during prolonged therapy with hydrALAZINE even though the patient is asymptomatic A positive antinuclear antibody titer requires that the physician carefully weigh the implications of the test results against the benefits to be derived from antihypertensive therapy with hydrALAZINE Laboratory tests such as complete blood count (CBC), electrocardiogram and serum creatinine or urea nitrogen may be indicated depending on the clinical situation, and periodic rechecking of the CBC and ANA may be helpful in early detection of untoward reactions

Medications that can induce a positive Antinuclear Antibody (ANA) test:

  • Hydralazine 2
  • Procainamide 3

Key points:

  • Both hydralazine and procainamide can induce a positive ANA test.
  • Patients should be monitored for signs of autoimmune disorders when taking these medications.
  • The decision to continue therapy should be weighed against the potential benefits and risks.

From the Research

Medications that Induce a Positive ANA Test

Medications can induce a positive Antinuclear Antibody (ANA) test, which is a common marker for autoimmune disorders such as Systemic Lupus Erythematosus (SLE). The following medications have been associated with a positive ANA test:

  • Hydralazine 4, 5, 6, 7, 8
  • Procainamide 4, 5, 7, 8
  • Isoniazid 4, 5, 7, 8
  • Methyldopa 4, 7
  • Chlorpromazine 4, 7
  • Quinidine 4, 7
  • Minocycline 4, 8
  • Calcium channel blockers 5, 8
  • Angiotensin-converting enzyme inhibitors 5, 8
  • Thiazide diuretics 5, 8
  • Terbinafine 5, 8
  • Tumor necrosis factor (TNF)-alpha antagonists 5, 8
  • Fluorouracile agents 8
  • Interferons 5, 7
  • Beta-blockers 7
  • Sulfonamides 7
  • Sulfasalazine 7
  • Penicillamine 7

Mechanism of Action

The exact mechanism of how these medications induce a positive ANA test is not fully understood, but it is thought to be related to the interaction between the drug and DNA or histones, rendering them immunogenic 7. In some cases, it may be due to disruption of the cytokine network 7.

Clinical Presentation

The clinical presentation of drug-induced lupus can vary, but it is often characterized by arthralgia, myalgia, pleurisy, rashes, and fever, in association with antinuclear antibodies in the serum 7. More serious features of idiopathic lupus, such as nephritis and cerebral disease, are rare in drug-induced disease 7.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.