What lab workup is recommended after a second spontaneous miscarriage?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: June 10, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Research

After a second spontaneous miscarriage, a comprehensive laboratory workup is recommended to identify potential underlying causes, including complete blood count, thyroid function tests, antiphospholipid antibody testing, parental karyotyping, and testing for inherited thrombophilias. This evaluation is crucial in identifying treatable conditions that might contribute to recurrent pregnancy loss, such as thyroid dysfunction, antiphospholipid syndrome, or structural uterine abnormalities. Some key tests to consider include:

  • Complete blood count to rule out any underlying hematological disorders
  • Thyroid function tests (TSH, free T4) to assess for thyroid dysfunction, as supported by 1
  • Antiphospholipid antibody testing (anticardiolipin antibodies, lupus anticoagulant, and beta-2 glycoprotein) to diagnose antiphospholipid syndrome, a known cause of recurrent miscarriage, as discussed in 2 and 3
  • Parental karyotyping to detect chromosomal abnormalities, which can contribute to recurrent pregnancy loss, as mentioned in 1
  • Testing for inherited thrombophilias, such as Factor V Leiden and prothrombin gene mutations, to identify potential coagulation disorders, as suggested by 1 Additional tests may include:
  • Hemoglobin A1C to screen for diabetes
  • Prolactin levels to assess for any endocrine disorders
  • Assessment of uterine anatomy through hysteroscopy or sonohysterography to identify any structural abnormalities
  • Testing for antithyroid antibodies and natural killer cell activity, as recommended by 1 The timing of this evaluation is important, ideally conducted when the patient is not pregnant to obtain the most accurate results, as implied by 4. Early identification of these factors allows for targeted interventions in subsequent pregnancies, potentially improving outcomes, as supported by the most recent and highest quality study, 1.

References

Research

Recurrent miscarriage: causes, evaluation and management.

Postgraduate medical journal, 2015

Research

Antiphospholipid syndrome and recurrent miscarriages.

European journal of obstetrics, gynecology, and reproductive biology, 2001

Research

Diagnosing miscarriage.

Best practice & research. Clinical obstetrics & gynaecology, 2009

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.