What causes yellow urine due to Vitamin B (Vitamin B2, riboflavin) intake?

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Last updated: June 14, 2025View editorial policy

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From the Guidelines

Yellow urine due to Vitamin B2 (riboflavin) intake is a harmless side effect caused by the excess vitamin being filtered by the kidneys and excreted in urine, giving it a bright yellow or fluorescent appearance. This occurs when you consume riboflavin in amounts that exceed what your body needs, typically at doses of 25-100 mg found in B-complex vitamins or multivitamins 1. The intensity of the yellow color directly correlates with the amount of riboflavin consumed.

Some key points to consider:

  • Riboflavin is water-soluble, meaning your body doesn't store much of it, so any amount beyond what's needed for metabolic processes is eliminated through urine 1.
  • The yellow color typically fades as the vitamin is cleared from your system, usually within several hours to a day after taking the supplement.
  • If you're concerned about the appearance, you could consider taking smaller doses or splitting your supplement throughout the day, though this isn't medically necessary.
  • The recommended daily dose of riboflavin is 1.3 mg for males and 1.1 mg for females, with higher doses recommended during pregnancy and lactation 1.

It's essential to note that while high doses of riboflavin (>100 mg) may have potential toxic effects, such as reacting with light to form potentially toxic peroxides, the typical doses found in supplements are generally safe and well-tolerated 1.

From the Research

Causes of Yellow Urine due to Vitamin B2 (Riboflavin) Intake

  • Yellow urine can be caused by the intake of Vitamin B2 (Riboflavin) due to its fluorescent properties 2.
  • Riboflavin is a water-soluble vitamin that is excreted in the urine, and high doses can cause the urine to turn yellow 2.
  • The yellow color is due to the presence of riboflavin and its metabolites in the urine, which can be detected by their fluorescent properties 3.

Mechanisms of Riboflavin Uptake and Excretion

  • Riboflavin uptake by cells is a carrier-mediated process that involves specific transporters such as RFVT-1, -2, and -3 4.
  • The uptake of riboflavin by pancreatic β-cells/islets is regulated by extracellular and intracellular factors, including a Ca(2+)/calmodulin-mediated regulatory pathway 4.
  • Riboflavin is excreted in the urine primarily through the kidneys, and its excretion can be influenced by various factors such as dietary intake, renal function, and individual variability 2.

Health Effects of Riboflavin Intake

  • Riboflavin has antioxidant properties and can protect against oxidative stress, which may contribute to its potential health benefits 3.
  • High intake of riboflavin has been associated with reduced risks of osteoporosis and bone loss in female adults 5.
  • However, excessive intake of riboflavin can cause adverse effects such as yellow urine, and high doses may interact with other medications or exacerbate certain health conditions 2.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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