What is the formula to calculate the Serum-Ascites Albumin Gradient (SAAG)?

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From the Guidelines

To calculate the Serum-Ascites Albumin Gradient (SAAG), subtract the albumin concentration in the ascitic fluid from the albumin concentration in the serum, using the formula: SAAG = Serum albumin - Ascitic fluid albumin, as recommended by the most recent guidelines 1. The process requires drawing a blood sample to measure serum albumin and performing a paracentesis to obtain ascitic fluid for albumin measurement, with both measurements taken on the same day for accuracy.

  • A SAAG value ≥1.1 g/dL indicates portal hypertension as the cause of ascites, such as cirrhosis, heart failure, or Budd-Chiari syndrome.
  • A value <1.1 g/dL suggests non-portal hypertensive causes, like malignancy, tuberculosis, or pancreatitis. This calculation is valuable because it helps differentiate the underlying cause of ascites more accurately than the traditional transudate-exudate classification, as supported by recent studies 1. The SAAG reflects the pressure gradient between the portal venous system and the ascitic fluid, making it physiologically relevant for determining the mechanism of ascites formation, and its interpretation is summarized in Table 4 of the 2021 practice guidance by the American Association for the Study of Liver Diseases 1.

From the Research

Serum-Ascites Albumin Gradient (SAAG) Formula

The formula to calculate the Serum-Ascites Albumin Gradient (SAAG) is:

  • SAAG = Serum albumin concentration - Ascitic fluid albumin concentration

SAAG Calculation and Interpretation

  • The SAAG is calculated by subtracting the ascitic fluid albumin concentration from the serum albumin concentration 2, 3, 4.
  • A high SAAG (≥1.1 g/dl) indicates portal hypertension, whereas a low SAAG (<1.1 g/dl) suggests no portal hypertension 2, 3, 4.
  • The SAAG is a useful marker for differentiating between causes of ascites, such as cirrhosis, heart failure, and malignant ascites 2, 4.

Clinical Applications of SAAG

  • The SAAG can be used to predict the presence of esophageal varices in patients with cirrhosis and ascites 3.
  • The SAAG is superior to the exudate-transudate concept in classifying ascites and identifying the underlying cause 4.
  • The SAAG has limited clinical implications in relation to the extent of portal hypertension in patients with liver cirrhosis 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

[Sero-ascitic gradient of albumin: usefulness and diagnostic limitations].

Anales de medicina interna (Madrid, Spain : 1984), 1995

Research

Serum-ascites albumin gradient in differential diagnosis of ascites.

Mymensingh medical journal : MMJ, 2013

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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