What is the mechanism of action of paracetamol (acetaminophen)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: June 24, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Paracetamol's mechanism of action is primarily through the inhibition of prostaglandin production within the central nervous system and peripheral tissues, as demonstrated by recent studies 1. The exact mechanism of paracetamol's analgesic action remains largely unknown, but it is believed to involve the inhibition of cyclooxygenase (COX) enzymes, particularly in the central nervous system.

  • Key aspects of paracetamol's mechanism include:
    • Inhibition of prostaglandin production, which contributes to its analgesic and antipyretic effects
    • Minimal effect on peripheral COX enzymes, resulting in limited anti-inflammatory action
    • Influence on the endocannabinoid system and serotonergic pathways, which may also contribute to its pain-relieving effects
  • The drug's ability to cross the blood-brain barrier effectively allows it to reach its central site of action, where it can inhibit prostaglandin production and exert its analgesic effects, as noted in the context of its use for various painful conditions 1.
  • It is essential to consider the potential risks associated with paracetamol use, including hepatotoxicity, particularly when taken in excessive amounts, as highlighted by studies examining its safety profile 1.

From the Research

Mechanism of Action of Paracetamol

The mechanism of action of paracetamol (acetaminophen) is complex and not fully understood. Several theories have been proposed, including:

  • Inhibition of cyclooxygenase (COX) enzymes in the central nervous system (CNS) 2, 3
  • Activation of the descending inhibitory serotonergic pathway 2
  • Potentiation of the endocannabinoid system through the formation of N-arachidonoylphenolamine (AM404) 4
  • Inhibition of prostaglandin synthesis in the brain 5
  • Activation of transient receptor potential vanilloid-1 (TRPV1) receptors 4

Key Findings

  • Paracetamol has been shown to inhibit COX-1 and COX-2 enzymes, although the exact mechanism of this inhibition is not fully understood 2, 3
  • The formation of AM404, a metabolite of paracetamol, has been proposed to activate the endocannabinoid system and contribute to the analgesic effects of paracetamol 4
  • Paracetamol has been shown to reduce the levels of prostaglandin E2 in the brain, which may contribute to its antipyretic effects 5
  • The antipyretic effect of paracetamol has been shown to occur independently of TRPA1-mediated hypothermia 5

Theories and Proposals

  • One theory proposes that paracetamol works as a pro-drug, with the active metabolite being a cannabinoid 4
  • Another proposal suggests that paracetamol may have a "redox" mechanism of action, although this is not fully understood 3
  • The exact mechanism of paracetamol's analgesic and antipyretic effects is still a topic of debate and research 2, 6, 3, 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Paracetamol: new vistas of an old drug.

CNS drug reviews, 2006

Research

Paracetamol: past, present, and future.

American journal of therapeutics, 2000

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.