What is the workup for hypercalcemia?

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From the Guidelines

The workup for hypercalcemia begins with confirming elevated calcium levels through repeat testing, including ionized calcium if available, and initial laboratory evaluation should include parathyroid hormone (PTH), phosphorus, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, creatinine, albumin, complete blood count, and urinary calcium excretion 1.

Initial Evaluation

The initial evaluation of hypercalcemia is crucial for determining the underlying cause. Key laboratory tests include:

  • Parathyroid hormone (PTH) to differentiate between primary hyperparathyroidism and other causes of hypercalcemia
  • Phosphorus, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D to assess vitamin D metabolism and phosphate homeostasis
  • Creatinine and albumin to evaluate renal function and calcium binding proteins
  • Complete blood count to rule out hematologic malignancies
  • Urinary calcium excretion to assess for hypercalciuria

Further Evaluation Based on PTH Levels

If PTH is elevated, primary hyperparathyroidism is likely, warranting parathyroid imaging with ultrasound and sestamibi scan 1.

  • Parathyroid imaging is essential for localizing the parathyroid adenoma or hyperplasia
  • Ultrasound and sestamibi scan are the preferred imaging modalities due to their high sensitivity and specificity

If PTH is suppressed, evaluate for malignancy with:

  • Chest X-ray to rule out lung cancer
  • Mammography in women to rule out breast cancer
  • Age-appropriate cancer screening to rule out other malignancies
  • Serum/urine protein electrophoresis to detect multiple myeloma Consider PTH-related protein measurement if malignancy is suspected 1.

Medication Review and Treatment

Medication review is essential as thiazide diuretics, lithium, and excessive vitamin D or calcium supplements can cause hypercalcemia 1. For severe hypercalcemia (>14 mg/dL) or symptomatic patients, immediate treatment with:

  • IV fluids to correct dehydration and enhance renal calcium excretion
  • Calcitonin to rapidly lower calcium levels
  • Bisphosphonates (like zoledronic acid 4mg IV or pamidronate 60-90mg IV) to inhibit bone resorption
  • Possibly hemodialysis for severe, life-threatening hypercalcemia may be necessary while completing the diagnostic workup 1.

The systematic approach is important because hypercalcemia has diverse etiologies, with primary hyperparathyroidism and malignancy accounting for approximately 90% of cases 1.

From the Research

Workup for Hypercalcemia

The workup for hypercalcemia involves several steps to determine the underlying cause of the condition.

  • Measurement of serum intact parathyroid hormone (PTH) is the first step to further evaluate confirmed hypercalcemia 2.
  • The PTH level helps to distinguish between PTH-dependent and PTH-independent causes of hypercalcemia 3.
  • An elevated or normal PTH concentration is consistent with primary hyperparathyroidism (PHPT), while a suppressed PTH level indicates another cause 3.

Diagnostic Tests

The following diagnostic tests may be used to evaluate hypercalcemia:

  • Serum calcium and PTH concentrations to establish a differential diagnosis 4.
  • Measurement of 1,25-dihydroxyvitamin D and PTH-related peptide to help identify the underlying cause of hypercalcemia 2.
  • Imaging tests such as ultrasound and sestamibi scan to identify parathyroid gland abnormalities 5.
  • Laboratory tests to rule out other causes of hypercalcemia, such as malignancy and granulomatous diseases 6, 2.

Approach to Diagnosis

The approach to diagnosis involves:

  • A careful history to identify potential causes of hypercalcemia, such as medication use or family history of hypercalcemia 2.
  • Physical examination to identify signs and symptoms of hypercalcemia, such as fatigue, constipation, and nausea 3.
  • Laboratory tests and imaging studies to confirm the diagnosis and identify the underlying cause of hypercalcemia 3, 4, 6, 2, 5.

Special Considerations

In some cases, hypercalcemia may be caused by multiple factors, such as primary hyperparathyroidism and malignancy-associated hypercalcemia 4.

  • A comprehensive workup, including laboratory and imaging tests, is necessary to exclude common non-parathyroid causes of hypercalcemia 5.
  • Genetic mutations, such as a mutation in the PTH gene, may be responsible for undetectable PTH levels in some cases of primary hyperparathyroidism 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

[Differential diagnosis of hypercalcemia in adults].

Medizinische Klinik (Munich, Germany : 1983), 2000

Research

Hypercalcemia: A Review.

JAMA, 2022

Research

Primary Hyperparathyroidism With Undetectable Intact Parathyroid Hormone.

Clinical medicine insights. Endocrinology and diabetes, 2024

Research

Hypercalcemia: an evidence-based approach to clinical cases.

Iranian journal of kidney diseases, 2009

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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