What are the uses for methylene blue in medical practice?

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Last updated: June 30, 2025View editorial policy

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From the Guidelines

Methylene blue is a crucial medication in medical practice, primarily used as a first-line treatment for methemoglobinemia, administered at 1-2 mg/kg intravenously, as supported by recent evidence 1.

Uses of Methylene Blue

  • Treatment of methemoglobinemia: Methylene blue acts by reducing methemoglobin to oxyhemoglobin in erythrocytes, with a usual starting dose of 1–2 mg/kg infused intravenously over 3 to 5 minutes 1.
  • Surgical settings: It functions as a vital dye for tissue visualization, particularly in sentinel lymph node mapping during cancer surgeries and for identifying urinary tract structures.
  • Treatment of vasoplegic syndrome: Methylene blue is used at doses of 1-2 mg/kg followed by continuous infusion if needed, to increase vascular tone in hypotensive conditions.
  • Antimicrobial properties: It is used to treat urinary tract infections and as a topical antiseptic.

Administration and Precautions

  • The dose may be repeated at 1 mg/kg if methemoglobinemia does not significantly decrease within 30–60 minutes, with a risk of worsening methemoglobinemia with repeated doses 1.
  • Patients with G6PD deficiency are at risk for complications after methylene blue administration, including hemolytic anemia, and should be tested for G6PD deficiency before treatment 1.
  • Methylene blue should be used with caution in pregnant women, patients with renal failure, and anesthetized patients, due to concerns about teratogenicity, hemolysis, and inhibition of guanylate cyclase 1.

Mechanism of Action

  • Methylene blue reduces methemoglobin by providing an alternative electron acceptor, creates visible staining through its blue color properties, and inhibits nitric oxide synthase to increase vascular tone in hypotensive conditions.
  • Potential side effects include blue discoloration of skin and urine, hemolytic anemia in G6PD-deficient patients, and serotonin syndrome when combined with certain psychiatric medications 1.

From the FDA Drug Label

PACKAGE LABEL.PRINCIPAL DISPLAY PANEL NDC 70771-1768-1 Methylene Blue Injection, USP 50 mg/10 mL (5 mg/mL) Intravenous use only Single-Dose vial Rx only The FDA drug label does not answer the question.

From the Research

Medical Uses of Methylene Blue

Methylene blue has several uses in medical practice, including:

  • Treatment of acquired methemoglobinemia (MetHB) 2
  • Treatment of ifosfamide neurotoxicity 2, 3, 4
  • Treatment of refractory vasoplegic shock in pediatric and adult patients 2, 5
  • Diagnostic tool in surgical procedures 3
  • Potential treatment for distributive shock, including sepsis and anaphylaxis 6
  • Treatment of ifosfamide-induced encephalopathy (IIE) 3, 4

Mechanism of Action and Side Effects

Methylene blue's mechanism of action is complex and based partly on its oxidizing capabilities 2. It can also act as a selective inhibitor of the NO-cGMP pathway, which plays a role in the pathophysiology of distributive shock 6. However, methylene blue can also induce serotonin syndrome, especially when used in conjunction with other serotonergic agents 5, 4.

Specific Applications

Methylene blue has been used in various areas of medicine, including:

  • Pediatric emergency care 2
  • Cardiac surgery 5
  • Oncology 4
  • Treatment of Alzheimer's disease and memory improvement 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Suspected serotonin syndrome in a patient being treated with methylene blue for ifosfamide encephalopathy.

Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners, 2012

Research

Methylene blue for distributive shock: a potential new use of an old antidote.

Journal of medical toxicology : official journal of the American College of Medical Toxicology, 2013

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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