What bacteria are not killed by hand rub (alcohol-based hand rub)?

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Last updated: March 5, 2025View editorial policy

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From the Guidelines

Bacterial spores, such as those from Clostridium spp. or Bacillus spp., are not reliably killed by alcohol-based hand rubs. This is because the spores have a protective coating that prevents the alcohol from penetrating and denaturing the proteins or disrupting the cell membrane 1. As a result, handwashing with soap and water is recommended for removing these spores from the surface of contaminated hands. Key points to consider include:

  • The use of alcohol-based hand rubs with at least 60-95% alcohol concentration
  • Applying enough product to cover all hand surfaces
  • Rubbing until completely dry, which takes about 20-30 seconds
  • Following facility-specific protocols in healthcare settings where resistant organisms are common
  • Proper technique is crucial, as inadequate volume or insufficient contact time significantly reduces effectiveness. In the case of outbreaks of C. difficile-related infections, washing hands with soap and water after removing gloves is prudent 1. Additionally, healthcare workers with suspected or documented exposure to B. anthracis-contaminated items should wash their hands with soap and water.

From the Research

Bacteria Not Killed by Hand Rub

  • Clostridium difficile spores are not effectively killed by alcohol-based hand rubs 2, 3, 4
  • Studies have shown that hand washing with soap and water is more effective in removing C. difficile spores from hands than alcohol-based hand rubs 2, 3, 5, 4
  • The use of alcohol-based hand rubs may not significantly reduce the transfer of C. difficile spores through physical contact 3

Effectiveness of Hand Hygiene Methods

  • Hand washing with soap and water has been shown to be superior to alcohol-based hand rubs in removing C. difficile spores from hands 2, 3, 5, 4
  • Chlorhexidine soap washing has also been shown to be effective in reducing C. difficile spore counts on hands 3
  • Proper hand hygiene technique is crucial in reducing the transmission of C. difficile and other nosocomial infections 5, 6

Importance of Hand Hygiene

  • Non-compliance with hand hygiene among medical staff contributes to nosocomial infections, which are a major cause of disease complications and deaths 6
  • Proper hand hygiene is a key measure for reducing the transmission of C. difficile and other nosocomial infections 5, 6

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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