What are the guidelines for testosterone replacement therapy (TRT) in patients with diagnosed hypogonadism?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: August 6, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Guidelines for Testosterone Replacement Therapy in Hypogonadism

Testosterone replacement therapy (TRT) should be initiated in men with confirmed hypogonadism based on at least two morning testosterone measurements below normal range (<300 ng/dL) along with clinical symptoms, with careful monitoring for efficacy and adverse effects. 1

Diagnosis Criteria

  • Diagnosis requires:
    • At least two morning testosterone measurements confirming low levels (total testosterone <300 ng/dL)
    • Presence of clinical symptoms including:
      • Decreased libido
      • Erectile dysfunction
      • Reduced energy levels
      • Loss of muscle mass
      • Increased body fat
      • Decreased bone mineral density
      • Depressive symptoms

Treatment Options

Available Formulations

  1. Topical gels (1% or 1.62%)

    • Starting dose for 1.62% gel: 40.5 mg (2 pump actuations) applied daily to shoulders and upper arms 2
    • Dose adjustment range: 20.25 mg to 81 mg based on serum testosterone levels 2
    • Apply to clean, dry skin; avoid application to abdomen, genitals, chest, armpits 3
  2. Injectable testosterone

    • Testosterone cypionate or enanthate
    • Peak levels occur 2-5 days post-injection
    • Trough levels return to baseline by 10-14 days 1
  3. Other formulations

    • Testosterone patches
    • Implantable pellets
    • Buccal tablets

Monitoring Protocol

Initial Monitoring

  • Measure serum testosterone at approximately 14 days and 28 days after starting treatment 2
  • Target testosterone range: 350-750 ng/dL 2

Ongoing Monitoring

  • Every visit: Check for:

    • Hematocrit/hemoglobin (risk of erythrocytosis)
    • PSA for prostate cancer risk
    • Symptom response
    • Digital rectal examination
    • Voiding symptoms
    • Sleep apnea symptoms 1
  • Dose Adjustment Guidelines:

    • If testosterone >750 ng/dL: Decrease daily dose by 20.25 mg
    • If testosterone 350-750 ng/dL: Continue current dose
    • If testosterone <350 ng/dL: Increase daily dose by 20.25 mg 2

Contraindications

Absolute Contraindications

  • Prostate cancer
  • Male breast cancer
  • Desire for fertility in the near future 1

Relative Contraindications

  • Severe obstructive sleep apnea (may not be absolute per recent evidence) 4
  • Severe lower urinary tract symptoms (may not be absolute per recent evidence) 4
  • Uncontrolled congestive heart failure
  • Hematocrit >54%
  • "Age-related hypogonadism" without structural or genetic etiology 2, 3

Benefits of TRT

TRT can improve:

  • Sexual function and libido
  • Quality of life
  • Lean body mass
  • Reduce abdominal subcutaneous adipose tissue
  • Improve insulin sensitivity in men with metabolic syndrome
  • Reduce inflammatory markers
  • Small improvements in depressive symptoms 1
  • Bone health and muscle strength in frail men 4

Potential Risks and Complications

  • Cardiovascular effects: Mixed evidence regarding cardiovascular risk; careful monitoring needed especially in men with pre-existing cardiovascular disease 1
  • Erythrocytosis: Monitor hematocrit; consider temporarily withholding therapy, reducing dose, or performing phlebotomy if hematocrit rises above reference range 1
  • Fertility impairment: Exogenous testosterone suppresses spermatogenesis 1
  • Prostate effects: Monitor PSA; consider urologic referral if PSA increases >1.0 ng/dL in first 6 months or >0.4 ng/dL per year thereafter 1
  • Secondary exposure: Risk of virilization in women and children who contact application sites; patients should wash hands thoroughly after application and cover application sites with clothing 2, 3

Common Pitfalls to Avoid

  • Diagnosing hypogonadism based on a single testosterone measurement
  • Failing to measure free testosterone in obese patients
  • Overlooking secondary causes of hypogonadism
  • Initiating therapy without proper baseline evaluation
  • Not monitoring for adverse effects, particularly hematocrit elevation
  • Using testosterone for "age-related hypogonadism" without structural or genetic etiology 1
  • Ignoring symptoms and overreacting to single lab values 1
  • High discontinuation rates observed in clinical practice - patients should be educated about the need for long-term therapy 5

TRT is an effective treatment for properly diagnosed hypogonadism, but requires careful patient selection, appropriate formulation choice based on patient preference, and vigilant monitoring to maximize benefits while minimizing risks.

References

Guideline

Testosterone Therapy Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.