What is the mechanism of action (MOA) of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)?

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Mechanism of Action of NSAIDs

NSAIDs exert their therapeutic effects primarily through inhibition of cyclooxygenase (COX) enzymes, which blocks prostaglandin synthesis in peripheral tissues, resulting in anti-inflammatory, analgesic, and antipyretic effects. 1, 2

COX Enzyme Inhibition

  • NSAIDs inhibit two structurally distinct forms of the cyclooxygenase enzyme:

    • COX-1: Constitutively expressed in normal cells, responsible for physiological functions
    • COX-2: Primarily induced in inflammatory cells 2
  • The inhibition of these enzymes leads to decreased production of prostaglandins, which are mediators of:

    • Inflammation
    • Pain sensitization (by sensitizing afferent nerves)
    • Fever
    • Various physiological functions 3, 4

Types of NSAIDs Based on COX Selectivity

  1. Traditional/Non-selective NSAIDs:

    • Reversibly inhibit both COX-1 and COX-2 activity
    • Examples: ibuprofen, naproxen, diclofenac 2, 1
    • Naproxen has the lowest cardiovascular risk profile 1
  2. COX-2 Selective Inhibitors (Coxibs):

    • Preferentially inhibit COX-2 over COX-1 due to higher affinity
    • Example: celecoxib (currently the only available coxib in the US) 2, 5
    • Designed to reduce gastrointestinal complications while maintaining anti-inflammatory effects 5
  3. Aspirin:

    • Unique in that it irreversibly blocks COX enzymes
    • Has cardioprotective effects not seen with other NSAIDs 2

Pharmacological Effects

  • Anti-inflammatory effects: Primarily through COX-2 inhibition, reducing production of inflammatory prostaglandins 4

  • Analgesic effects: By decreasing prostaglandin synthesis, which:

    • Reduces sensitization of afferent nerves
    • Diminishes the action of bradykinin in inducing pain 3
  • Antipyretic effects: Through inhibition of prostaglandin synthesis in the central nervous system 6

Clinical Implications of COX Inhibition

  • Gastrointestinal effects: COX-1 inhibition reduces protective prostaglandins in the GI tract, leading to potential ulceration and bleeding 2, 1

  • Cardiovascular effects: COX-2 inhibition without balanced COX-1 inhibition may increase cardiovascular risks by altering the thromboxane/prostacyclin balance 7

  • Renal effects: Both COX-1 and COX-2 are constitutively expressed in the kidney; inhibition can lead to sodium retention, reduced renal blood flow, and potential acute kidney injury in susceptible individuals 8

  • Platelet effects: COX-1 inhibition reduces thromboxane A2 production, affecting platelet aggregation 1

Important Clinical Considerations

  • The therapeutic benefit of NSAIDs comes from their anti-inflammatory properties, which provide superior pain control compared to simple analgesics like acetaminophen 2

  • COX-2 selective inhibitors were developed to maintain anti-inflammatory effects while reducing gastrointestinal toxicity, but may have increased cardiovascular risks 5, 7

  • Both COX-1 and COX-2 have physiological roles in various tissues, explaining the diverse side effect profile of NSAIDs 8, 9

Understanding this mechanism of action helps explain both the therapeutic benefits and adverse effects of NSAIDs, guiding appropriate clinical use and risk assessment for individual patients.

References

Guideline

NSAID Therapy Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Mechanism of action of aspirin-like drugs.

Seminars in arthritis and rheumatism, 1997

Research

NSAIDs and cardiovascular risk.

Journal of cardiovascular medicine (Hagerstown, Md.), 2017

Research

Mechanism of action of nonsteroidal anti-inflammatory drugs.

The Veterinary clinics of North America. Small animal practice, 2000

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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