Can Wellbutrin (bupropion) cause a false positive on a urine drug screen?

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Wellbutrin (Bupropion) Can Cause False Positive Urine Drug Screens for Amphetamines

Bupropion hydrochloride extended-release tablets (Wellbutrin) can cause false-positive urine test results for amphetamines, as explicitly stated in the FDA drug label. 1

Mechanism and Frequency of False Positives

Bupropion is a commonly prescribed antidepressant that appears to be the most frequent cause of false positive urine drug screens for amphetamines in some populations:

  • Studies show that 41% of false positive amphetamine screens were associated with bupropion use 2
  • The false positive result occurs with certain immunoassay tests, particularly:
    • Syva EMIT II immunoassay 2
    • Certain enzyme-linked immunosorbent assay (ELISA) tests 3
    • CEDIA assays (which can also show false positives for LSD) 4

Detection Thresholds and Metabolites

  • False positive results typically occur when bupropion metabolites are present in concentrations greater than 500 ng/mL 3
  • It's primarily the metabolites of bupropion, rather than the parent drug itself, that cross-react with the amphetamine screening tests 3

Confirmatory Testing

When a positive amphetamine screen occurs in a patient taking Wellbutrin:

  • Confirmatory testing using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS) is essential 5
  • These confirmatory methods can differentiate between true amphetamine use and bupropion-induced false positives 6
  • Without confirmation, patients may face unwarranted consequences, including potential legal or employment issues

Clinical Implications and Best Practices

  1. Documentation is crucial: Always document any medications the patient is taking, including Wellbutrin/bupropion, to help interpret drug screen results 5

  2. Patient communication: Discuss unexpected positive results with patients rather than taking immediate punitive action 5

  3. Laboratory collaboration: Establish a working relationship with laboratory toxicologists to assist with result interpretation 5

  4. Awareness of other medications: Be aware that other commonly prescribed medications can also cause false positives on urine drug screens, including:

    • Antihistamines (brompheniramine, diphenhydramine)
    • Other antidepressants (sertraline, trazodone, venlafaxine)
    • Antipsychotics (quetiapine, chlorpromazine)
    • Over-the-counter medications containing pseudoephedrine 5, 7

Common Pitfalls to Avoid

  • Failing to order confirmatory testing when a patient on bupropion tests positive for amphetamines
  • Not informing patients taking Wellbutrin about the possibility of false positive drug screens
  • Dismissing patients from care based solely on unconfirmed positive urine drug test results 5
  • Assuming that all drug screening methods have the same cross-reactivity with bupropion

By understanding that Wellbutrin can cause false positive amphetamine results and following appropriate confirmatory testing protocols, clinicians can avoid misinterpretation of drug screening results and prevent potential harm to patients.

References

Research

Frequency of false positive amphetamine screens due to bupropion using the Syva EMIT II immunoassay.

Journal of medical toxicology : official journal of the American College of Medical Toxicology, 2011

Guideline

Substance Use Screening Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Quantitation of Bupropion in Urine by Liquid Chromatography-Tandem Mass Spectrometry.

Methods in molecular biology (Clifton, N.J.), 2024

Research

Commonly prescribed medications and potential false-positive urine drug screens.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists, 2010

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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