What is the use of fasting C-peptide (Connecting Peptide) levels?

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From the Guidelines

Fasting C-peptide levels are primarily used to distinguish between type 1 and type 2 diabetes, particularly in ambiguous cases, and to assess pancreatic beta cell function 1. When interpreting fasting C-peptide levels, it is essential to consider the clinical context and the presence of hyperglycemia.

  • Low C-peptide levels indicate decreased insulin production, suggesting type 1 diabetes.
  • Normal or elevated levels in the presence of hyperglycemia suggest insulin resistance as seen in type 2 diabetes. The measurement of C-peptide can aid in differentiating between type 1 and type 2 diabetes, especially when the clinical presentation is unclear 1. Additionally, fasting C-peptide levels can be useful in diagnosing insulinomas and investigating suspected factitious hypoglycemia, as they are not affected by exogenous insulin administration 1. It is recommended to measure fasting C-peptide level when simultaneous fasting plasma glucose is £220 mg/dL (12.5 mmol/L), particularly if required by the payer for coverage of insulin pump therapy 1. Overall, fasting C-peptide testing provides valuable information for determining appropriate diabetes treatment strategies and assessing pancreatic function.

From the Research

Use of Fasting C-peptide Levels

  • Fasting C-peptide levels are used to measure endogenous insulin secretion in patients with diabetes 2, 3, 4, 5, 6
  • C-peptide is produced in equal amounts to insulin and is a reliable indicator of pancreatic beta-cell function 2, 4, 5
  • Measurement of fasting C-peptide levels can help differentiate between type 1 and type 2 diabetes 2, 3, 4, 6
  • Fasting C-peptide levels can also be used to predict the need for insulin therapy in patients with type 2 diabetes, although its ability to do so is limited 3
  • A low fasting C-peptide level can indicate absolute insulin deficiency and the need for insulin treatment, while a high level may suggest insulin resistance 4, 5

Clinical Applications

  • Fasting C-peptide levels can be used to assist in the diagnosis and classification of diabetes, including latent autoimmune diabetes in adults (LADA) and maturity-onset diabetes of the young (MODY) 4
  • C-peptide measurement can help identify patients with type 1 diabetes who are likely to experience partial remission during the first year of treatment 4
  • Fasting C-peptide levels can also be used to monitor the effectiveness of treatments aimed at preserving insulin secretion, such as islet cell transplantation 4, 5

Interpretation of Results

  • A fasting C-peptide level of less than 0.2 nmol/l is associated with a diagnosis of type 1 diabetes mellitus (T1DM) 5
  • The fasting C-peptide/glucose ratio (CGR) may be a useful indicator of endogenous insulin secretion and can aid in differential diagnosis and treatment decisions 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

The clinical utility of C-peptide measurement in the care of patients with diabetes.

Diabetic medicine : a journal of the British Diabetic Association, 2013

Research

Fasting C-peptide and Related Parameters Characterizing Insulin Secretory Capacity for Correctly Classifying Diabetes Type and for Predicting Insulin Requirement in Patients with Type 2 Diabetes.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2016

Research

[The clinical utility of C-peptide measurement in diabetology].

Pediatric endocrinology, diabetes, and metabolism, 2015

Research

A Practical Review of C-Peptide Testing in Diabetes.

Diabetes therapy : research, treatment and education of diabetes and related disorders, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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