Are Novolog (insulin aspart) and insulin lispro (Humalog) the same?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: August 25, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Novolog and Insulin Lispro: Different Rapid-Acting Insulin Analogs

No, Novolog (insulin aspart) and insulin lispro (Humalog) are not the same insulin product, though they are both rapid-acting insulin analogs with very similar pharmacokinetic and pharmacodynamic profiles.

Differences Between Insulin Aspart and Insulin Lispro

Insulin aspart (Novolog) and insulin lispro (Humalog) are distinct rapid-acting insulin analogs that differ in their molecular structure:

  • Molecular structure: Each has different amino acid modifications to the insulin molecule 1

    • Insulin aspart: Proline at position B28 is replaced with aspartic acid
    • Insulin lispro: Proline at position B28 and lysine at position B29 are reversed
  • Manufacturer:

    • Insulin aspart (Novolog): Manufactured by Novo Nordisk
    • Insulin lispro (Humalog): Manufactured by Eli Lilly

Similarities in Clinical Properties

Despite their structural differences, these insulins share remarkably similar clinical properties:

  • Onset of action: Both have onset within 15-30 minutes 2
  • Peak action: Both peak at approximately 1-3 hours 2
  • Duration of action: Both last approximately 3-5 hours 2
  • Timing of administration: Both should be administered immediately before meals 2

Comparative Efficacy and Clinical Use

Research has shown that insulin aspart and insulin lispro have virtually indistinguishable clinical effects:

  • A direct comparison study found that both insulins produced similar serum insulin levels (250-300 pmol/l) at approximately 30 minutes and disappeared from serum after approximately 4 hours 3

  • Both insulins had equivalent effects on:

    • Glucose uptake
    • Glucose oxidation
    • Endogenous glucose production
    • Lipid metabolism 3
  • Some studies suggest minor differences in absorption rates, with one study indicating insulin aspart may have slightly faster absorption compared to insulin lispro 4, though this has not translated to meaningful clinical differences 5

Clinical Applications

Both insulin aspart and insulin lispro are used in similar clinical scenarios:

  • Basal-bolus regimens: Both are used as the bolus (mealtime) component 2
  • Insulin pump therapy: Both are suitable for continuous subcutaneous insulin infusion (CSII) 2
  • Dosing: Initial dosing recommendations are similar (0.1-0.2 units/kg per meal or 4 units per meal) 2

Important Clinical Considerations

  • Interchangeability: While pharmacologically similar, these insulins are not identical products and should not be automatically substituted without provider awareness

  • Prescription specificity: Prescriptions should specify which rapid-acting analog is intended

  • Patient education: Patients should be taught to recognize their specific insulin by both brand and generic name to avoid medication errors

  • Insurance considerations: Insurance formularies may cover one product but not the other, potentially affecting patient access and costs 2

Conclusion

While insulin aspart (Novolog) and insulin lispro (Humalog) are different insulin products with distinct molecular structures, their clinical profiles are remarkably similar. For most patients, the choice between these two rapid-acting insulin analogs would have minimal impact on glycemic control outcomes.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Insulin Therapy Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Differences in pharmacokinetics and pharmacodynamics of insulin lispro and aspart in healthy volunteers.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2002

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.