What is the procedure for an Oral Glucose Tolerance Test (OGTT)?

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From the Guidelines

The procedure for an Oral Glucose Tolerance Test (OGTT) involves fasting for at least 8 hours, consuming a 75g glucose solution, and then measuring blood glucose levels at fasting, 1 hour, and 2 hours after consumption, as recommended by the most recent guidelines 1. The patient should fast for 8-12 hours overnight and have a baseline blood sample drawn to measure fasting glucose. Next, the patient drinks a sweet solution containing 75 grams of glucose (for adults) or a dose based on body weight for children. Blood samples are then collected at specific intervals, typically at 1 hour, 2 hours, and sometimes 3 hours after drinking the glucose solution. Throughout the test, the patient remains seated and avoids eating, drinking (except water), smoking, or physical activity, as these can affect results. The test evaluates how efficiently the body processes glucose and is commonly used to diagnose diabetes, gestational diabetes, and prediabetes, with diagnostic criteria including fasting plasma glucose, 1-hour plasma glucose, and 2-hour plasma glucose values 1. It works by challenging the body's insulin response - in healthy individuals, blood glucose rises after consuming the glucose solution but returns to normal within 2 hours as insulin helps cells absorb the glucose, while in those with impaired glucose metabolism, levels remain elevated for longer periods. The most recent guidelines from 2023 provide the best evidence for the procedure and diagnostic criteria for OGTT 1, which is more reliable than older studies 1. Key points to consider when performing an OGTT include:

  • Fasting period: at least 8 hours
  • Glucose dose: 75g for adults
  • Blood sample collection: fasting, 1 hour, and 2 hours after glucose consumption
  • Diagnostic criteria: based on plasma glucose values at different time points 1.

From the Research

Procedure for Oral Glucose Tolerance Test (OGTT)

The Oral Glucose Tolerance Test (OGTT) is a diagnostic tool used to assess the body's ability to regulate blood glucose levels after consuming a sugary drink. The procedure involves:

  • Preparing for the test by following specific dietary instructions and avoiding certain medications 2
  • Fasting for a certain period before the test, usually overnight 3
  • Consuming a 75g oral glucose load, usually in the form of a sugary drink 2, 4
  • Measuring venous plasma glucose levels before and 2 hours after the glucose load 2, 5
  • Interpreting the results based on the glucose levels measured at these two time points 2, 4

Key Considerations

  • The OGTT is considered the gold standard for diagnosing diabetes 2
  • The test can also be used to identify individuals with impaired glucose tolerance (IGT), which is a precursor to type 2 diabetes 5, 4
  • The results of the OGTT can be affected by various factors, including diet, physical activity, and certain medications 3
  • Recent studies have explored the use of near-patient glucometers for measuring fasting glucose levels before the OGTT, which can provide faster results but may be less accurate than laboratory-based measurements 6

Administration and Interpretation

  • The OGTT should be administered by a healthcare professional in a clinical setting 2, 3
  • The results of the test should be interpreted based on established criteria, including the glucose levels measured at the two time points 2, 4
  • The test can provide valuable information about an individual's glucose metabolism and can be used to diagnose and monitor diabetes and other related conditions 5, 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Oral glucose tolerance testing.

Australian family physician, 2012

Research

The oral glucose tolerance test (OGTT) revisited.

European journal of internal medicine, 2011

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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