Is CA-125 (Cancer Antigen 125) the best blood test for detecting ovarian cancer?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: September 4, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

CA-125 is Not the Best Blood Test for Detecting Ovarian Cancer

CA-125 alone is not the best blood test for detecting ovarian cancer due to its limited sensitivity and specificity, especially in early-stage disease where only about 50% of cases produce enough CA-125 to cause a positive test. 1

Limitations of CA-125 as a Standalone Test

  • Limited sensitivity in early disease: CA-125 has approximately 50% sensitivity in stage I ovarian cancer cases, though it improves to 80-85% sensitivity in stage II or greater disease 2

  • Poor specificity: CA-125 levels can be elevated in numerous non-cancerous conditions, including:

    • Benign gynecologic conditions: endometriosis, adenomyosis, pelvic inflammatory disease, menstruation, uterine fibroids, and benign ovarian cysts 2
    • Non-gynecologic conditions: peritonitis, cirrhosis, pancreatic cancer, and conditions causing pleural effusion or ascites 2
    • Other cancers: breast, lung, colon, and pancreatic cancer 2
  • Population limitations: CA-125 has higher specificity in postmenopausal women compared to premenopausal women 2

Better Approaches Than CA-125 Alone

Multimodal Screening Approaches

  • CA-125 + Transvaginal Ultrasound (TVU): The UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) assessed a multimodal screening strategy that included annual CA-125 screening using a risk of ovarian cancer algorithm (ROCA) with TVU as a second-line test. This approach was more effective at detecting early-stage ovarian cancer than either modality alone 1

  • Combined biomarkers: The combination of CA-125 and Human Epididymis Protein 4 (HE4) has proven highly efficient with an area under the curve (AUC) of up to 0.96, making it currently the most efficient biological diagnostic tool for ovarian cancer 3

Algorithmic Approaches

  • Risk of Ovarian Cancer Algorithm (ROCA): This algorithm uses age and longitudinal changes in CA-125 levels over time rather than single measurements, which may be more effective than a single CA-125 measurement 1

  • ROMA (Risk of Ovarian Malignancy Algorithm): Combines HE4 and CA-125 measurements, though its specificity (84%) is lower than HE4 alone (94%) 3

Current Recommendations

  • No professional organization recommends CA-125 alone for screening average-risk women for ovarian cancer 1

  • The American College of Obstetricians and Gynecologists (ACOG) and the Society of Gynecologic Oncology (SGO) recommend referral to a gynecologic oncologist for any elevation of CA-125, particularly levels greater than 200 units/mL 2

  • Pattern of CA-125 elevation (progressive increases over time) is more concerning for malignancy than a single elevated value 2

Pitfalls and Caveats

  • Using CA-125 alone for screening can lead to unnecessary interventions, surgeries, and psychological harm due to false positives 4

  • CA-125 results must be interpreted in the context of the patient's complete clinical picture, including menopausal status and presence of other conditions that could elevate the marker 2

  • The high performance of CA-125 reported in some studies may be due to recruitment bias, restriction to postmenopausal women, and inclusion of only primary invasive epithelial ovarian cancer cases 5

In conclusion, while CA-125 remains widely used in ovarian cancer evaluation, it is most valuable when combined with other biomarkers (particularly HE4) and imaging modalities (transvaginal ultrasound) rather than as a standalone test for detecting ovarian cancer.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Ovarian Cancer Diagnosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Highly accurate detection of ovarian cancer using CA125 but limited improvement with serum matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 2010

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.