What is the mechanism of action of Ozempic (semaglutide)?

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Mechanism of Action of Ozempic (Semaglutide)

Semaglutide, the active ingredient in Ozempic, is a GLP-1 receptor agonist that selectively binds to and activates the GLP-1 receptor, reducing blood glucose primarily through glucose-dependent stimulation of insulin secretion and suppression of glucagon secretion. 1

Primary Mechanism of Action

Semaglutide works through several key mechanisms:

  • GLP-1 Receptor Activation: Semaglutide is a GLP-1 analogue with 94% sequence homology to human GLP-1 that selectively binds to and activates the GLP-1 receptor 1

  • Glucose-Dependent Effects on Pancreatic Hormones:

    • Stimulates insulin secretion when blood glucose is high
    • Suppresses glucagon secretion in a glucose-dependent manner
    • Preserves counter-regulatory glucagon response during hypoglycemia 1
  • Delayed Gastric Emptying: Causes a delay in early postprandial gastric emptying, reducing the rate at which glucose appears in circulation after meals 1, 2

Molecular Structure and Modifications

Semaglutide has been specifically engineered for extended duration of action through several modifications:

  • Modified at position 8 to provide stabilization against degradation by dipeptidyl peptidase-4 (DPP-4) enzyme 1

  • Modified at position 26 lysine with a hydrophilic spacer and a C18 fatty di-acid to facilitate albumin binding 1

  • Minor modification at position 34 to ensure attachment of only one fatty di-acid 1

  • These modifications result in:

    • Decreased renal clearance
    • Protection from metabolic degradation
    • Extended half-life of approximately 1 week 1

Physiological Effects

Semaglutide treatment results in several measurable physiological changes:

  • Glucose Reduction: In patients with type 2 diabetes, semaglutide 1 mg reduces:

    • Fasting glucose by 29 mg/dL (22%)
    • 2-hour postprandial glucose by 74 mg/dL (36%)
    • Mean 24-hour glucose concentration by 30 mg/dL (22%) 1
  • Effects on Glucagon: Treatment with semaglutide results in:

    • 8% reduction in fasting glucagon
    • 14-15% reduction in postprandial glucagon response
    • 12% reduction in mean 24-hour glucagon concentration 1
  • Insulin Secretion: Both first- and second-phase insulin secretion are increased in patients with type 2 diabetes treated with semaglutide 1

Gastrointestinal Effects

  • GLP-1 receptor activation delays gastric emptying by inhibiting gastric peristalsis while increasing pyloric tone 2

  • This delay is more pronounced in people with normal rapid gastric emptying 2

  • The mechanism appears to be mediated by the vagus nerve, as effects on gastric emptying are not present in patients who have had a vagotomy 2

Clinical Implications

The mechanism of action of semaglutide translates to several important clinical benefits:

  • Improved glycemic control without increased risk of hypoglycemia due to its glucose-dependent action 2, 1

  • Significant weight reduction through appetite suppression and delayed gastric emptying 3

  • Cardiovascular benefits demonstrated in multiple clinical trials 2

Important Considerations

  • The long half-life of approximately 1 week means that effects persist for an extended period after discontinuation 1

  • Gastrointestinal side effects (nausea, vomiting, diarrhea) are common due to the delay in gastric emptying 3

  • The drug should be discontinued at least 2 months before a planned pregnancy due to its long washout period 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Obesity Management with GLP-1 Receptor Agonists

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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