What are the side effects of methotrexate?

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Last updated: September 16, 2025View editorial policy

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Methotrexate Side Effects

Methotrexate has numerous potential side effects ranging from common gastrointestinal symptoms to serious and potentially life-threatening complications affecting multiple organ systems, with the most concerning being bone marrow suppression, hepatotoxicity, and pulmonary toxicity.

Common Side Effects

Gastrointestinal

  • Nausea and vomiting (occurs in up to 25% of patients, typically within 12-24 hours after dosing) 1
  • Abdominal distress
  • Diarrhea
  • Stomatitis and oral ulcers
  • Anorexia

General

  • Fatigue and malaise
  • Headache
  • Dizziness

Serious Adverse Effects

Hepatotoxicity

  • Transient elevation of liver enzymes (common)
  • Fibrosis and cirrhosis (with long-term use) 1, 2
  • Risk factors for hepatotoxicity include:
    • Obesity (BMI ≥ 40 kg/m²)
    • Diabetes
    • Hyperlipidemia
    • Greater than moderate alcohol consumption
    • Pre-existing liver disease (hepatitis B/C)
    • Family history of inheritable liver disease 1

Hematologic Toxicity

  • Leukopenia
  • Thrombocytopenia
  • Pancytopenia (rare but potentially fatal)
  • Anemia
  • Increased risk with:
    • Renal insufficiency
    • Advanced age
    • Drug interactions
    • Hypoalbuminemia
    • Alcohol intake 1, 2

Pulmonary Toxicity

  • Interstitial pneumonitis (can be acute or chronic)
  • Pulmonary fibrosis
  • Respiratory failure
  • Symptoms include dry, nonproductive cough, dyspnea, fever, and hypoxemia 1, 2
  • Risk factors include:
    • Pre-existing lung disease
    • Cigarette smoking
    • Advanced age 3

Renal Effects

  • Reduced renal function increases methotrexate toxicity
  • Acute renal failure (particularly with high doses)
  • Dose adjustment required for impaired renal function:
    • GFR > 90 mL/min: Normal dose
    • GFR 20-50 mL/min: Half dose
    • GFR < 20 mL/min: Avoid methotrexate 1

Dermatologic

  • Photosensitivity
  • Skin ulceration
  • Erythematous rashes
  • Alopecia
  • Severe reactions (rare): toxic epidermal necrolysis, Stevens-Johnson syndrome 2

Neurologic

  • Headaches
  • Drowsiness
  • Blurred vision
  • Cognitive dysfunction
  • Leukoencephalopathy (with high doses or intrathecal administration) 2

Reproductive

  • Teratogenic effects (contraindicated in pregnancy)
  • Temporary oligospermia in men
  • Women should wait at least 3 months after discontinuation before attempting conception
  • Men should wait 3 months after discontinuation before attempting to father a child 1

Drug Interactions

Medications that may increase methotrexate toxicity include:

  • NSAIDs (salicylates, naproxen, ibuprofen)
  • Antibiotics (trimethoprim/sulfamethoxazole, penicillins, sulfonamides)
  • Probenecid
  • Certain diuretics (furosemide, thiazides) 1, 3

Monitoring Recommendations

  • Complete blood count, liver function tests, and renal function should be monitored:
    • Every 2-4 weeks initially
    • Every 3-6 months once stable on therapy 1
  • Laboratory tests should be performed 4-6 days after dosing (not immediately after) 1
  • Patients with risk factors for hepatotoxicity should have annual non-invasive liver fibrosis assessment 1

Risk Mitigation

  • Folic acid supplementation (1-5 mg daily except on the day of methotrexate) reduces nausea, stomatitis, and hepatic adverse effects 1
  • Taking medication before bedtime or with food may reduce nausea 1
  • Antiemetics (ondansetron, granisetron) can help manage nausea 1
  • Parenteral (subcutaneous) administration may reduce gastrointestinal side effects 1
  • Complete abstinence from alcohol is advised during therapy 3

Special Considerations

  • Pediatric patients generally tolerate methotrexate well with appropriate monitoring 1
  • Elderly patients and those with renal impairment require closer monitoring and potentially dose reduction
  • Test dose (1.25-5 mg) may be considered in patients with risk factors for toxicity 1

By understanding these potential side effects and implementing appropriate monitoring, many adverse events can be detected early and managed effectively, allowing patients to benefit from methotrexate therapy while minimizing risks.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

High-Dose Methotrexate Administration

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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