What dose of vitamin B6 (pyridoxine) should be administered with HRZE (rifampicin, isoniazid, pyrazinamide, ethambutol) therapy?

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Last updated: September 17, 2025View editorial policy

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Vitamin B6 (Pyridoxine) Dosing with HRZE Therapy

Pyridoxine (vitamin B6) should be given at a dose of 25-50 mg daily to all persons at risk of neuropathy when receiving HRZE therapy, and the dose should be increased to 100 mg daily for patients who develop peripheral neuropathy. 1

Standard Dosing Recommendations

For Prevention of Neuropathy:

  • Standard preventive dose: 25-50 mg daily 1, 2
  • For intermittent dosing regimens: 50-100 mg twice weekly 1

For Treatment of Established Neuropathy:

  • Treatment dose: 100 mg daily 1, 2

Risk Factors Requiring Pyridoxine Supplementation

Pyridoxine supplementation is indicated for all patients with the following risk factors:

  • Pregnant women
  • Breastfeeding mothers
  • Persons with HIV infection
  • Patients with diabetes
  • Alcoholism
  • Malnutrition
  • Chronic renal failure
  • Advanced age 1, 2

Mechanism and Rationale

Isoniazid (INH), a key component of HRZE therapy, interferes with pyridoxine metabolism by:

  • Competitively inhibiting pyridoxine's action in metabolic functions 3
  • Causing reduction in glutamic-oxaloacetic transaminase (GOT) activity 4
  • Leading to deficiency of both coenzyme (pyridoxal phosphate) and apoenzyme 4

This interference can result in peripheral neuropathy, characterized by:

  • Initial symptoms of burning feet 5
  • Paresthesia
  • Distal sensory impairment that may extend proximally
  • Loss of deep tendon reflexes

Duration of Supplementation

Pyridoxine supplementation should be continued for the entire duration of anti-TB therapy containing isoniazid 2.

Special Considerations

Dosing Based on Acetylator Status:

  • Slow inactivators of isoniazid are at higher risk of developing peripheral neuropathy 6, 4
  • Consider measuring acetylator status if symptoms develop, and potentially reducing isoniazid dose to 3 mg/kg/day or less in slow acetylators 5

Safety Concerns:

  • Long-term high-dose supplementation (>500 mg/day) can cause sensory neuropathy 2
  • Even doses as low as 100 mg/day have been associated with Lhermitte signs (spinal cord effects) with long-term use 2
  • Symptoms of toxicity include sensory neuropathy with ataxia, areflexia, impaired sensations, and dermatologic lesions 2

Cost Considerations

In resource-limited settings, even low-dose pyridoxine (6 mg daily) has been shown to be effective in preventing peripheral neuropathy in patients receiving high-dose isoniazid 6, 4. This makes prophylaxis feasible even in developing countries.

Monitoring

If peripheral neuropathy develops despite prophylaxis, consider:

  1. Increasing pyridoxine dose to 100 mg daily 1, 2
  2. Evaluating for slow acetylator status 5
  3. Potentially reducing isoniazid dose if appropriate 5

By following these guidelines for pyridoxine supplementation, the risk of isoniazid-induced peripheral neuropathy can be significantly reduced while maintaining the effectiveness of HRZE therapy for tuberculosis.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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