What are the causes of pseudohematuria?

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Causes of Pseudohematuria

Pseudohematuria refers to red or discolored urine that mimics hematuria but does not contain actual red blood cells. It is an important differential diagnosis when evaluating patients with apparent blood in the urine 1, 2.

Common Causes of Pseudohematuria

Food and Dietary Sources

  • Beetroot consumption
  • Foods with red or purple pigments
  • Blackberries
  • Rhubarb

Medications

  • Mesalazine (can react with toilet bowl cleaners containing bleach) 3
  • Rifampin
  • Phenazopyridine
  • Doxorubicin
  • Phenolphthalein (in laxatives)
  • Nonsteroidal anti-inflammatory drugs (NSAIDs) 4

Metabolic Conditions

  • Porphyria
  • Hemoglobinuria (from intravascular hemolysis) 5
  • Myoglobinuria (from rhabdomyolysis)

Chemical Reactions

  • Reaction between medications and toilet bowl cleaners 3
  • Highly alkaline urine

Distinguishing Pseudohematuria from True Hematuria

Key Diagnostic Tests

  • Urinalysis with microscopy: Absence of RBCs despite positive dipstick for blood suggests pseudohematuria 2
  • Urine dipstick test patterns:
    Finding True Hematuria Hemoglobinuria Myoglobinuria Other Pigments
    Dipstick Positive Positive Positive Negative
    RBCs on microscopy Present Absent Absent Absent
    Plasma color Normal Pink/red Normal Normal
    Urine sediment Red Clear red Clear red/brown Varies

Clinical Approach to Suspected Pseudohematuria

  1. Detailed medication history

    • Focus on medications known to cause urine discoloration
    • Ask specifically about NSAIDs, which have been implicated in up to 54% of cases of idiopathic hematuria 4
  2. Dietary history

    • Recent consumption of foods with strong pigments
  3. Laboratory evaluation

    • Complete urinalysis with microscopy (to confirm absence of RBCs)
    • Urine culture (to rule out infection)
    • If hemoglobinuria is suspected: serum haptoglobin, LDH, and peripheral blood smear
  4. Consider specialized testing for specific conditions

    • Flow cytometry for paroxysmal nocturnal hemoglobinuria if hemoglobinuria is present 5
    • Urine porphobilinogen for porphyria

Important Clinical Considerations

  • Pseudohematuria must be distinguished from true hematuria, as the latter requires thorough evaluation for potentially serious conditions including malignancy 6, 7
  • The American Urological Association recommends that patients with true microscopic hematuria (>3 RBC/HPF) undergo risk stratification and appropriate evaluation 1, 6
  • Hemoglobinuria is often misdiagnosed as hematuria, leading to unnecessary urologic workup 5
  • In patients taking mesalazine for inflammatory bowel disease, be aware that a chemical reaction with toilet bowl cleaners can cause alarming urine discoloration 3

Pitfalls to Avoid

  • Assuming all red urine contains blood without microscopic confirmation
  • Failing to take a thorough medication and dietary history
  • Overlooking hemoglobinuria as a cause of red urine in patients with hemolytic conditions
  • Missing the distinction between glomerular and non-glomerular sources of true hematuria when it is present
  • Conducting extensive urologic workups before ruling out pseudohematuria

By systematically evaluating patients with discolored urine and confirming the presence or absence of actual red blood cells, clinicians can avoid unnecessary testing while ensuring appropriate management for patients with true hematuria.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Hematuria. When is it cause for alarm?

Postgraduate medicine, 1987

Research

Pseudohaematuria Due to Mesalazine: A Case Report.

Nephrology (Carlton, Vic.), 2025

Guideline

Urinary Tract Infection Diagnosis and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Hematuria.

Primary care, 2019

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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