What is the dosage and administration of colistin for treating multidrug-resistant gram-negative bacterial infections?

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Colistin Dosage and Administration for Multidrug-Resistant Gram-Negative Bacterial Infections

For treating multidrug-resistant gram-negative bacterial infections, colistin should be administered with a loading dose of 5 mg/kg of colistin base activity (CBA) IV, followed by a maintenance dose of 2.5 mg CBA × (1.5 × CrCl + 30) IV every 12 hours, with appropriate renal function adjustments. 1

Dosage Recommendations

Standard Dosing

  • Loading dose: 5 mg/kg of colistin base activity (CBA) IV 1
  • Maintenance dose: 2.5 mg CBA × (1.5 × CrCl + 30) IV every 12 hours 1
  • Dosage equivalence: 1 million IU of colistin ≈ 33 mg of colistin base activity (CBA) or 80 mg of colistimethate sodium (CMS) 1

Renal Adjustment

Dosage should be modified based on renal function:

Degree of Renal Impairment Creatinine Clearance (mL/min) Dosage Schedule
Normal ≥80 2.5 to 5 mg/kg, divided into 2 to 4 doses per day
Mild 50-79 2.5 to 3.8 mg/kg, divided into 2 doses per day
Moderate 30-49 2.5 mg/kg, once daily or divided into 2 doses per day
Severe 10-29 1.5 mg/kg every 36 hours

2

Administration Methods

Intravenous Administration

Two options for IV administration:

  1. Direct Intermittent Administration:

    • Slowly inject one-half of the total daily dose over 3-5 minutes every 12 hours 2
  2. Continuous Infusion:

    • Inject one-half of the total daily dose over 3-5 minutes
    • Add the remaining half to compatible IV solutions (0.9% NaCl, 5% dextrose, etc.)
    • Administer by slow IV infusion over the next 22-23 hours 2

Reconstitution

  • The 150 mg vial should be reconstituted with 2 mL Sterile Water for Injection
  • This provides a concentration equivalent to 75 mg/mL colistin base activity 2

Treatment Duration

Infection Type Recommended Duration
Complicated UTI 5-10 days
Complicated intra-abdominal infections 5-10 days
VAP/HAP 10-14 days
Bacteremia 10-14 days

1

Combination Therapy Considerations

For multidrug-resistant Acinetobacter baumannii (CRAB) infections:

  • Avoid colistin-carbapenem combination therapy (strong recommendation against use; high certainty of evidence) 3
  • Avoid colistin-rifampin combination therapy (strong recommendation against use, moderate certainty of evidence) 3
  • For severe CRAB infections, consider combination therapy with two in vitro active antibiotics among available options (polymyxin, aminoglycoside, tigecycline, sulbactam combinations) 3
  • For CRAB with meropenem MIC <8 mg/L, consider carbapenem combination therapy with high-dose extended-infusion carbapenem dosing 3

Efficacy and Safety Considerations

Efficacy

  • Higher colistin doses (median 2.9 mg/kg/day vs 1.5 mg/kg/day) have been associated with improved microbiological success and 7-day survival 4
  • Clinical response rates of 73-85% have been reported in studies using appropriate dosing regimens 5, 6

Safety Monitoring

  • Nephrotoxicity: Close monitoring of renal function is essential as colistin can cause nephrotoxicity in 8-30% of patients 1
  • Higher doses (median 3.8 mg/kg/day vs 1.6 mg/kg/day) have been associated with increased risk of acute kidney injury 4
  • Monitor renal function regularly throughout treatment 1

Important Considerations

  • In obese patients, dosing should be based on ideal body weight 2
  • For critically ill patients, infectious disease consultation is strongly recommended 1
  • The broth microdilution method is the reference standard for colistin susceptibility testing per CLSI and EUCAST guidelines 7
  • Colistin should be reserved for infections proven or strongly suspected to be caused by susceptible bacteria due to its potential toxicity 1

Clinical Pearls

  • Higher colistin doses are associated with better microbiological clearance but may increase nephrotoxicity risk, requiring careful balance 4
  • Any final IV infusion solution containing colistimethate sodium should be freshly prepared and used within 24 hours 2
  • Reconstituted solution for intramuscular injection should be refrigerated and used within 7 days 2

References

Guideline

Treatment of XDR Pseudomonas aeruginosa Infections

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Association between colistin dose and microbiologic outcomes in patients with multidrug-resistant gram-negative bacteremia.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2013

Research

Effectiveness and nephrotoxicity of intravenous colistin for treatment of patients with infections due to polymyxin-only-susceptible (POS) gram-negative bacteria.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2006

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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