Why are methotrexate and folic acid (Vitamin B9) given together?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: October 3, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Why Methotrexate and Folic Acid Are Given Together

Folic acid supplementation is strongly recommended for all patients on methotrexate therapy to reduce toxicity without compromising treatment efficacy. 1

Mechanism and Rationale

  • Methotrexate is a folate antagonist that inhibits folate-dependent purine and pyrimidine synthesis pathways, which is part of its therapeutic mechanism but also the source of many side effects 1
  • Folic acid supplementation helps replenish folate stores depleted by methotrexate, reducing adverse effects while maintaining therapeutic efficacy 1, 2
  • The supplementation specifically targets the unwanted toxicity without interfering with methotrexate's beneficial anti-inflammatory effects 1, 3

Benefits of Folic Acid Supplementation

Reduction in Side Effects

  • Hepatotoxicity: Folic acid significantly reduces the risk of liver enzyme elevations by up to 76.9% (relative risk reduction) 4, 5
  • Gastrointestinal side effects: 26% relative risk reduction in nausea, vomiting, and abdominal pain 5
  • Stomatitis and mucosal effects: Trend toward reduction in mouth sores, though not always statistically significant 5
  • Overall toxicity: Reduces the likelihood of methotrexate discontinuation by 60.8% 5

Maintained Efficacy

  • Multiple studies have shown that folic acid supplementation does not significantly reduce methotrexate's therapeutic efficacy in treating psoriasis or rheumatoid arthritis 1, 2, 4
  • Daily folic acid supplementation appears safe and does not interfere with methotrexate's clinical benefits 2

Dosing Recommendations

  • Dosage: 1-5 mg of folic acid daily is the standard recommendation 1
  • Timing: Typically given daily except on the day(s) of methotrexate administration to avoid potential interference with efficacy 1
  • Minimum recommendation: At least 5 mg of folic acid per week is strongly recommended by rheumatology guidelines 1
  • Alternative: Folinic acid (leucovorin) can also be used but is more expensive with similar efficacy 1, 4

Important Clinical Considerations

  • Folic acid is preferred over folinic acid for routine supplementation due to lower cost and similar effectiveness 1, 6
  • Folinic acid should be reserved for treating acute methotrexate toxicity rather than routine prevention 1, 3
  • Lack of folate supplementation is a major risk factor for methotrexate-induced hematologic toxicity 1
  • The FDA drug label for methotrexate acknowledges that vitamin preparations containing folic acid may decrease responses to systemically administered methotrexate, but clinical evidence shows the benefits outweigh this theoretical concern 7, 3

Pitfalls to Avoid

  • Timing error: Administering folinic acid too close to methotrexate dosing may reduce methotrexate efficacy, unlike folic acid which can be given more flexibly 2
  • Underdosing: Insufficient folate supplementation may fail to prevent toxicity 1
  • Discontinuation: Stopping folic acid supplementation during ongoing methotrexate therapy increases risk of adverse effects 6
  • Monitoring lapse: Even with folate supplementation, regular monitoring of liver function, blood counts, and renal function remains essential 1

The evidence strongly supports that folic acid supplementation should be a standard practice for all patients receiving methotrexate therapy, as it significantly reduces toxicity without compromising therapeutic efficacy 1, 3, 4.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.