What to do about a pap smear showing atypical squamous cells that cannot exclude high-grade squamous intraepithelial lesion (HSIL)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: October 9, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Management of Pap Smear with Atypical Squamous Cells Cannot Exclude HSIL (ASC-H)

Immediate colposcopy is strongly recommended for patients with ASC-H on Pap smear due to the high risk (up to 50%) of underlying high-grade cervical intraepithelial neoplasia (CIN 2,3). 1

Initial Management

  • Colposcopy is the recommended first-line management for all women with ASC-H, as endorsed by the American Society for Colposcopy and Cervical Pathology (ASCCP) guidelines 1
  • HPV testing alone is not recommended for initial triage of ASC-H, unlike with ASC-US, as the prevalence of high-grade lesions is significantly higher 1
  • If colposcopy results are negative or unsatisfactory, endocervical assessment using a cytobrush or endocervical curette is preferred 1
  • For non-pregnant patients aged ≥25 years with ASC-H and positive HPV 16, expedited treatment may be considered as an alternative to colposcopy 1

Follow-up After Colposcopy

  • If colposcopy is negative (no lesions identified), follow-up should include either:
    • Repeat cytology at 6 and 12 months, OR
    • HPV testing at 12 months 1
  • Colposcopic reevaluation is indicated if follow-up HPV testing is positive or if repeat cytology shows ASC-US or greater 1
  • Even with a negative colposcopy after ASC-H, patients require close surveillance as their risk of being diagnosed with follow-up cervical abnormalities is significantly above baseline 2

Special Considerations

Age-Related Differences

  • Premenopausal women with ASC-H have a higher likelihood (65%) of having high-grade lesions compared to postmenopausal women (35%) 3
  • Postmenopausal women with ASC-H have a lower predictive value (22.5%) for high-grade lesions compared to pregnant women (79.6%), postpartum women (66.7%), and contraceptive users (60%) 4
  • For postmenopausal women with ASC-H, HPV testing may be beneficial to better identify those at risk for high-grade lesions before proceeding to colposcopy 3

HIV-Infected Women

  • HIV-infected women with ASC-H should undergo colposcopy and directed biopsy 1
  • More frequent cervical screening is recommended for HIV-infected women with a history of abnormal Pap tests 1
  • After treatment for cervical dysplasia, HIV-infected women require careful follow-up with frequent cytologic screening and colposcopic examination when indicated due to increased risk of recurrence 1

Clinical Significance and Outcomes

  • Studies show that ASC-H represents approximately 0.26-0.3% of all Pap tests 3, 5
  • The risk of underlying high-grade disease with ASC-H is substantial:
    • 2.9% for invasive cervical cancer
    • 1.7% for adenocarcinoma in situ (AIS)
    • 65.6% for HSIL 5
  • A negative HPV test in the context of ASC-H has been found to have an excellent negative predictive value for HSIL in some studies, suggesting potential utility in reducing unnecessary colposcopies 6

Common Pitfalls and Caveats

  • Do not dismiss or delay evaluation of ASC-H as it carries a significant risk of underlying high-grade disease 5
  • Avoid relying solely on HPV testing for initial management of ASC-H, as current guidelines recommend immediate colposcopy 1
  • Be aware that even with negative colposcopy results after ASC-H, patients remain at elevated risk for subsequent cervical abnormalities and require continued surveillance 2
  • Consider age and hormonal status when interpreting the clinical significance of ASC-H, as the predictive value varies significantly between premenopausal and postmenopausal women 3, 4

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.