What is the administration protocol for Tenecteplase (tissue plasminogen activator) in myocardial infarction (MI)?

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Last updated: October 12, 2025View editorial policy

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Administration Protocol for Tenecteplase in Myocardial Infarction

Tenecteplase should be administered as a single intravenous bolus over 5 seconds with weight-adjusted dosing as follows: 30 mg if <60 kg, 35 mg if 60-<70 kg, 40 mg if 70-<80 kg, 45 mg if 80-<90 kg, and 50 mg if ≥90 kg. 1, 2

Timing and Indications

  • Initiate treatment as soon as possible after the onset of STEMI symptoms to maximize benefits 1
  • Tenecteplase is indicated to reduce mortality risk in acute ST-elevation myocardial infarction (STEMI) 1
  • Treatment should be given within 12 hours of symptom onset; greatest benefit occurs with earlier administration 3
  • Consider tenecteplase when primary PCI cannot be performed by an experienced team within 120 minutes of first medical contact 2
  • For patients presenting very early (<2 hours after symptom onset) with a large infarct and low bleeding risk, fibrinolysis should be considered if time from first medical contact to balloon inflation is >90 minutes 2

Preparation and Administration

  • Reconstitute the 50 mg vial with 10 mL of sterile water for injection to achieve a concentration of 5 mg/mL 1
  • Gently swirl until completely dissolved; do not shake 1
  • Determine appropriate dose based on patient's weight and withdraw this volume from reconstituted vial 1
  • Inspect for particulate matter and discoloration before administration 1
  • Flush dextrose-containing IV lines with 0.9% sodium chloride before and after administration to prevent precipitation 1
  • Administer as a single IV bolus over 5 seconds 1

Weight-Based Dosing Table

Patient Weight (kg) Tenecteplase (mg) Volume to be administered (mL)
< 60 30 6
≥ 60 to < 70 35 7
≥ 70 to < 80 40 8
≥ 80 to < 90 45 9
≥ 90 50 10

2, 1

Adjunctive Therapy

  • Aspirin: Administer oral loading dose of 150-300 mg or IV dose of 80-150 mg if oral ingestion not possible 2
  • Clopidogrel: Indicated in addition to aspirin 2
  • Anticoagulation: Required until revascularization (if performed) or for duration of hospital stay up to 8 days 2
  • Preferred anticoagulant options:
    • Enoxaparin: 30 mg IV bolus followed by 1.0 mg/kg subcutaneous injection every 12 hours (preferred over UFH in patients <75 years without significant renal dysfunction) 2
    • Unfractionated heparin (UFH): 60 U/kg IV bolus (maximum 4000 U) followed by 12 U/kg/hour infusion (maximum 1000 U/hour), adjusted to maintain aPTT at 1.5-2.0 times control (approximately 50-70 seconds) 2

Post-Administration Monitoring

  • Monitor ST-segment elevation, cardiac rhythm, and clinical symptoms over 60-180 minutes after initiation of therapy 2
  • Signs of successful reperfusion include:
    • Relief of symptoms
    • Maintenance or restoration of hemodynamic and/or electrical stability
    • Reduction of at least 50% of initial ST-segment elevation on follow-up ECG 60-90 minutes after initiation 2
  • Monitor platelet counts daily in patients given UFH 2
  • Transfer to a PCI-capable center following fibrinolysis is indicated in all patients 2
  • Consider rescue PCI immediately if fibrinolysis has failed (<50% ST-segment resolution at 60 minutes) 2

Contraindications

  • Active internal bleeding 1
  • History of cerebrovascular accident 1
  • Intracranial or intraspinal surgery or trauma within 2 months 1
  • Intracranial neoplasm, arteriovenous malformation, or aneurysm 1
  • Known bleeding diathesis 1
  • Severe uncontrolled hypertension 1

Special Considerations

  • In patients with known heparin-induced thrombocytopenia, consider bivalirudin as an alternative to heparin 2
  • Avoid tenecteplase in patients >75 years when using LMWH as ancillary therapy 2
  • Avoid LMWH in patients with significant renal dysfunction (serum creatinine >2.5 mg/dL in men or >2.0 mg/dL in women) 2
  • Tenecteplase has a longer half-life (22 minutes vs 3.5 minutes) compared to alteplase, allowing for single bolus administration 4
  • The combination of tenecteplase, aspirin, enoxaparin, and clopidogrel has been extensively studied as part of a pharmacoinvasive strategy 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Safety of TNKase Administration Outside the Approved Time Window

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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