At what postmenstrual age or chronological age should the first retinopathy of prematurity (ROP) screening examination be performed in newborns?

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Last updated: October 28, 2025View editorial policy

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Timing of First ROP Screening Examination in Newborns

The first retinopathy of prematurity (ROP) screening examination should be performed at the later of either 31-33 weeks postmenstrual age or 4 weeks chronological age. 1

Screening Criteria Based on Gestational Age and Birth Weight

  • Infants requiring ROP screening include those with gestational ages of 30 6/7 weeks or less (regardless of birth weight) and birth weights of 1250 g or less (regardless of gestational age) 2
  • Some centers may choose to extend birth weight screening criteria to 1500 g based on local factors 2
  • Infants with more than 37 weeks of gestation at birth do not need to be screened 1
  • Infants between 29 and 37 weeks may not need screening if they had a "medically stable" course (no supplemental oxygen requirement) 1
  • A more conservative approach is to screen all infants with less than 32 weeks of gestation at birth, even if medically stable 1

Timing of Initial Examination

  • For infants with gestational ages of 26 6/7 weeks or less at birth: Initial screening should be performed at 31 weeks postmenstrual age 2
  • For infants with gestational ages of 27 weeks or more at birth: Initial screening should be performed at 4 weeks chronological age 2
  • The examination should be conducted by an ophthalmologist experienced in the evaluation of ROP in premature infants 1

Evidence Supporting These Recommendations

  • Analysis of data from the CRYO-ROP study showed that in 99% of infants, retinal conditions indicating risk of poor outcome were not observed before 31 weeks postmenstrual age or 4 weeks chronological age 3
  • A recent study of extremely preterm infants (<27 weeks gestation) found that no infants required laser therapy for ROP prior to 32 weeks PMA, supporting the recommendation to initiate screening at 31 weeks PMA 4
  • Earlier studies had suggested that extremely low birth weight infants (<1000g) might benefit from earlier screening using the chronological age guideline of 4-6 weeks rather than waiting until 31-33 weeks postmenstrual age 5
  • However, more recent evidence confirms that even in extremely preterm infants, severe ROP requiring treatment does not develop before 31 weeks PMA 4, 6

Follow-up Examinations

  • Subsequent examinations are based on the findings at the first screening and should follow the ophthalmologist's recommendation 2
  • The mean interval between examinations is approximately 7-9 days 6
  • Screening can be discontinued when any of these signs is present 3:
    • Infant attains 45 weeks postmenstrual age without development of prethreshold ROP or worse
    • Progression of retinal vascularization into zone III without previous zone II ROP
    • Complete retinal vascularization

Special Considerations for Infants with Chronic Lung Disease

  • Infants with chronic lung disease of infancy (CLDI) rarely have a "medically stable" course during initial hospital weeks and should be screened even if between 29-37 weeks gestation 1
  • For infants with unresolved ROP and CLDI being discharged home, careful coordination of follow-up ophthalmology appointments is crucial 1
  • ROP that is regressing with vessels that have passed into Zone 3 on at least two sequential examinations is extremely unlikely to progress to threshold ROP 1
  • Infants whose vessels and/or ROP are still in Zone 1 or Zone 2 are at higher risk for progression to threshold and require ophthalmology visits every 1-2 weeks 1

Importance of Timely Screening

  • Early identification of prethreshold ROP is critical for providing timely intervention in this rapidly progressive disease 5
  • Peripheral ablation for threshold ROP has proven effective in reducing blindness from ROP 1
  • Missing follow-up appointments for infants still at risk for ROP progression can lead to missed opportunities for treatment and potentially preventable vision loss 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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