What is the initial workup and treatment for a patient with suspected myasthenia gravis?

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Initial Workup and Treatment for Suspected Myasthenia Gravis

The initial workup for suspected myasthenia gravis should include acetylcholine receptor (AChR) antibodies, anti-striated muscle antibodies, and if AChR antibodies are negative, test for muscle-specific kinase (MuSK) and lipoprotein-related protein 4 (LRP4) antibodies, followed by treatment with pyridostigmine as first-line therapy. 1, 2

Diagnostic Workup

Clinical Presentation Assessment

  • Look for fatigable or fluctuating muscle weakness, typically more proximal than distal, with possible ocular and/or bulbar involvement (ptosis, diplopia, dysphagia, dysarthria) 2, 3
  • Perform the ice test for ocular symptoms: apply ice pack over closed eyes for 2 minutes - highly specific for myasthenia gravis 2

Laboratory Testing

  • Order acetylcholine receptor (AChR) antibodies and anti-striated muscle antibodies 1, 4
  • If AChR antibodies are negative, test for muscle-specific kinase (MuSK) and lipoprotein-related protein 4 (LRP4) antibodies 1, 4
  • Consider measuring CPK, aldolase, ESR, and CRP to evaluate for concurrent myositis 4

Electrodiagnostic Studies

  • Perform single-fiber electromyography (SFEMG) - considered the gold standard with >90% sensitivity in ocular myasthenia 1, 3
  • Conduct repetitive nerve stimulation testing, though it's positive in only about one-third of patients with ocular myasthenia 1

Respiratory Function Assessment

  • Assess pulmonary function with negative inspiratory force (NIF) and vital capacity (VC) measurements 4
  • Apply the "20/30/40 rule" to identify patients at risk of respiratory failure: vital capacity <20 ml/kg, maximum inspiratory pressure <30 cmH₂O, or maximum expiratory pressure <40 cmH₂O 4
  • Perform single breath count test (counting at two numbers per second while exhaling) - counting to ≥25 correlates with normal respiratory function 4

Treatment Approach

First-Line Treatment

  • Start pyridostigmine (acetylcholinesterase inhibitor) at 30 mg orally three times daily 1, 2, 5
  • Gradually increase dose as needed for symptom control, up to maximum of 120 mg four times daily 1, 2
  • Be aware that approximately 50% of patients with ocular myasthenia may show minimal response to pyridostigmine alone 1, 2

Second-Line Treatment

  • For patients with Grade 2 or higher symptoms or inadequate response to pyridostigmine, add corticosteroids (prednisone 1-1.5 mg/kg/day) 1, 2
  • Corticosteroids are effective in approximately 66-85% of patients 1, 2
  • Gradually taper corticosteroid dose as symptoms improve 1

Third-Line/Additional Treatment Options

  • Consider azathioprine as an immunosuppressant for long-term treatment 1, 6
  • For moderate to severe symptoms not responding to initial therapy, consider plasmapheresis or IVIG (2 g/kg over 5 days) 2
  • Evaluate for thymectomy in appropriate patients 2

Management of Myasthenic Crisis

  • Immediately hospitalize with ICU-level monitoring 4
  • Continue corticosteroids and initiate either IVIG (2 g/kg over 5 days) or plasmapheresis (5 sessions over 5 days) 4
  • Perform daily neurological evaluations and frequent respiratory function assessments 4

Important Precautions

  • IMMEDIATELY discontinue medications that can worsen myasthenia gravis: 1, 4, 2
    • Beta-blockers
    • IV magnesium (absolutely contraindicated)
    • Fluoroquinolones
    • Aminoglycosides
    • Macrolide antibiotics

Follow-up and Monitoring

  • Regular assessment of respiratory function, especially in patients with generalized weakness 1, 4
  • Consider polysomnography when concerned about hypoventilation not captured by standard pulmonary function tests 4
  • Collaborative management between neurologist and ophthalmologist, particularly for patients with ocular involvement 1, 2

Special Considerations

  • Approximately 50-80% of patients with initial ocular symptoms may develop generalized myasthenia within a few years, requiring vigilant monitoring 2
  • For patients with refractory disease, newer therapies like efgartigimod alfa-fcab may be considered for AChR-positive patients 1, 7

References

Guideline

Tratamiento y Diagnóstico de la Miastenia Gravis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Diagnostic Criteria and Treatment Options for Myasthenia Gravis (MG)

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Myasthenia gravis.

Orphanet journal of rare diseases, 2007

Guideline

Management of Myasthenia Gravis Crisis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Treatment-refractory myasthenia gravis.

Journal of clinical neuromuscular disease, 2014

Research

Diagnosis and Management of Myasthenia Gravis.

Continuum (Minneapolis, Minn.), 2022

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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