What is the recommended frequency for routine testing for sexually transmitted infections (STIs)?

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Last updated: October 30, 2025View editorial policy

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Recommended Frequency for Routine STI Screening

All sexually active individuals should undergo routine STI screening based on their risk factors, with sexually active women ≤25 years and men who have sex with men (MSM) requiring annual screening at minimum, and higher-risk individuals needing more frequent testing every 3-6 months. 1

General Screening Recommendations by Population

Women

  • All sexually active women ≤25 years should be screened annually for chlamydia and gonorrhea regardless of reported risk behaviors 1
  • Women >25 years should be screened annually if they have risk factors (multiple partners, new partner, inconsistent condom use, substance use during sex, sex work) 1
  • Routine trichomoniasis screening is not recommended for asymptomatic adolescents but should be considered for women with high-risk behaviors 1

Men Who Have Sex with Men (MSM)

  • Annual screening for pharyngeal, rectal, and urethral gonorrhea and chlamydia is recommended for all sexually active MSM based on sexual practices 1, 2
  • More frequent screening (every 3-6 months) is recommended for MSM with higher risk factors (multiple or anonymous partners, sex with drug use) 1, 2
  • Annual syphilis screening is recommended for all sexually active MSM, with more frequent screening (every 3-6 months) for those at higher risk 1

Heterosexual Men

  • Routine screening is not universally recommended for heterosexual men without symptoms 1
  • Consider annual screening for sexually active young men in settings with high prevalence (≥2%) 1
  • Screen men with high-risk behaviors (multiple partners, inconsistent condom use, substance use during sex) 1

Risk-Based Screening Frequency

Higher Risk Individuals (requiring more frequent screening)

  • Screen every 3-6 months for individuals with:
    • Multiple or anonymous sexual partners 1, 3
    • Sex in conjunction with illicit drug use 1
    • Partners who engage in high-risk behaviors 1
    • History of recent STIs 1
    • Sex work or exchanging sex for money/drugs 1
    • Those using HIV pre-exposure prophylaxis (PrEP) 4, 5

Post-Treatment Rescreening

  • Rescreen all individuals 3 months after treatment for chlamydia or gonorrhea, regardless of partner treatment status 1
  • Consider rescreening females previously diagnosed with trichomoniasis 3 months after treatment 1
  • If 3-month rescreening is not possible, retest at next healthcare visit within 12 months 1

Site-Specific Screening Considerations

  • Collect specimens based on sexual practices and exposure sites 1, 2
  • For MSM, include pharyngeal swabs for those engaging in receptive oral sex 2
  • Include rectal swabs for individuals engaging in receptive anal intercourse 2, 6
  • Nucleic acid amplification tests (NAATs) are preferred for all specimen types due to higher sensitivity 2

Special Populations

HIV-Infected Individuals

  • Screen all HIV-infected individuals for syphilis at initiation of care and periodically thereafter based on risk 1
  • Screen all HIV-infected women for trichomoniasis and all women ≤25 years for chlamydia 1
  • Screen men and women for gonorrhea and chlamydia at initial presentation and annually if at risk 1
  • Retest in 3 months for those found positive for gonorrhea, chlamydia, or trichomoniasis due to high reinfection rates 1

Clinical Implications

  • Quarterly STI screening in high-risk populations can significantly reduce the number of asymptomatic infections that would otherwise go undetected and potentially be transmitted 5
  • Model simulations suggest that increasing screening frequency to biannually for all sexually active MSM could avert 72% of gonorrhea and 78% of chlamydia infections over 10 years 3
  • Self-collected samples may increase screening rates in populations with limited access to clinical settings 6

The evidence strongly supports tailoring STI screening frequency to individual risk factors, with annual screening as the minimum for most sexually active individuals and more frequent screening (every 3-6 months) for those with higher risk profiles.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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