What is the best post-exposure prophylaxis (PEP) regimen for a needle stick injury?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 1, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Best Post-Exposure Prophylaxis for Needle Stick Injury

For needle stick injuries with risk of HIV exposure, the preferred PEP regimen is bictegravir/emtricitabine/tenofovir alafenamide or dolutegravir plus (tenofovir alafenamide or tenofovir disoproxil fumarate) plus (emtricitabine or lamivudine), initiated as soon as possible and within 72 hours of exposure. 1

Initial Assessment and Timing

  • PEP should be initiated as soon as possible after exposure, ideally within 24 hours, but no later than 72 hours 1
  • The initial dose should not be delayed pending laboratory test results 1
  • A rapid HIV test or laboratory-based antigen/antibody combination test should be performed on the exposed individual before starting PEP 1

Risk Assessment

  • Evaluate the severity of exposure and likelihood of HIV transmission 1
  • Consider the HIV status of the source person (if known) and their viral suppression status 1
  • PEP is recommended when exposure to nonintact skin or mucous membranes presents a substantial risk for HIV transmission 1
  • Exposures to bodily fluids that don't pose significant risk (tears, non-blood-stained saliva, urine, sweat) don't require PEP 1

Recommended PEP Regimens

For Adults and Adolescents:

  • First-line regimen: Bictegravir/emtricitabine/tenofovir alafenamide OR dolutegravir plus (tenofovir alafenamide or tenofovir disoproxil fumarate) plus (emtricitabine or lamivudine) 1
  • Alternative backbone: Tenofovir disoproxil fumarate (TDF) + lamivudine (3TC) or emtricitabine (FTC) 1
  • Alternative third drugs: Lopinavir/ritonavir (LPV/r) or atazanavir/ritonavir (ATV/r) 1

For Children ≤10 years:

  • Preferred backbone: Zidovudine (ZDV) + lamivudine (3TC) 1
  • Preferred third drug: Lopinavir/ritonavir (LPV/r) 1
  • Alternative options: Abacavir + 3TC or TDF + 3TC/FTC (backbone); atazanavir/ritonavir, raltegravir, darunavir, efavirenz, or nevirapine (third drug) 1

Duration and Follow-up

  • The recommended PEP course is 28 days, regardless of exposure severity 1
  • A full 28-day prescription should be provided following initial risk assessment 1
  • Follow-up HIV antibody testing at 4-6 weeks and 3 months post-exposure 1
  • Enhanced adherence counseling is recommended for all individuals on PEP 1

Additional Prophylaxis Considerations

Hepatitis B Prophylaxis:

  • For unvaccinated individuals exposed to HBsAg-positive source: Hepatitis B Immune Globulin (HBIG) 0.06 mL/kg IM immediately and initiate HB vaccine series 2
  • For vaccinated individuals with inadequate antibody response: HBIG immediately plus HB vaccine booster 2

Other Considerations:

  • Evaluate for sexually transmitted infections if relevant to exposure 1
  • Consider hepatitis testing and vaccination as appropriate 1
  • Advise exposed individuals to use precautions to prevent secondary transmission during follow-up 1

Special Situations Requiring Expert Consultation

  • Delayed exposure report (beyond 24-36 hours) 1
  • Unknown source (e.g., needle in sharps container) 1
  • Pregnancy in the exposed person 1
  • Known or suspected resistance of source virus to antiretroviral agents 1
  • Toxicity of the initial PEP regimen 1

Common Pitfalls and Caveats

  • Do not delay PEP initiation while waiting for source person's HIV test results 1
  • Do not test needles or sharp instruments directly for HIV 1
  • PEP failure can occur despite appropriate regimen use, possibly due to drug resistance 3
  • PEP is unlikely to be effective when initiated more than 72 hours after exposure 1
  • Side effects like nausea and diarrhea are common but can often be managed without changing the PEP regimen 1

Resources for Consultation

  • National Clinicians' Post-Exposure Prophylaxis Hotline (PEPline): 1-888-448-4911 1
  • Local infectious disease specialists for complex cases 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.