Will Keflex (cephalexin) be effective in treating a urinary tract infection with a urine culture showing 50,000 Colony-Forming Units per milliliter (CFU/mL) of Lactobacillus species?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 4, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Keflex (Cephalexin) Will Not Work for Lactobacillus and Should Be Discontinued

Lactobacillus species at 50,000 CFU/mL does not represent a true urinary tract infection and should not be treated with antibiotics, including Keflex. 1

Why This Is Not a True UTI

Lactobacillus is explicitly identified as a contaminant, not a pathogen, in urine cultures. The American Academy of Pediatrics guidelines specifically state that "organisms such as Lactobacillus spp, coagulase-negative staphylococci, and Corynebacterium spp are not considered clinically relevant urine isolates for otherwise healthy" patients. 1

This represents either:

  • Vaginal/periurethral contamination during specimen collection 1
  • Normal urogenital flora that colonizes the distal urethra 1
  • Asymptomatic bacteriuria that should not be treated 1

Why Cephalexin Won't Work (And Shouldn't Be Used)

Even if treatment were indicated (which it is not), cephalexin has no meaningful activity against Lactobacillus species:

  • Lactobacillus are Gram-positive rods that are intrinsically resistant to most cephalosporins 2
  • Cephalexin's spectrum covers typical uropathogens like E. coli and Klebsiella, not Lactobacillus 2, 3
  • The high urinary concentrations achieved by cephalexin (500-1000 mcg/mL) are irrelevant when the organism is not susceptible 2

The Harm of Treating This

Treating asymptomatic bacteriuria or contaminants promotes antimicrobial resistance without clinical benefit. 4

Key risks include:

  • Unnecessary antibiotic exposure leading to resistance development 4
  • Disruption of protective vaginal flora, potentially increasing future UTI risk 1
  • Adverse effects including gastrointestinal symptoms and potential C. difficile infection 5
  • Misdiagnosis of the actual clinical problem if symptoms are present 1

What Should Be Done Instead

If the patient is symptomatic:

  • Reassess for true UTI with proper specimen collection technique (catheterized or midstream clean-catch) 1
  • Look for pyuria (≥5 WBC/hpf or positive leukocyte esterase) to distinguish true infection from contamination 1
  • Culture should grow ≥50,000 CFU/mL of a true uropathogen (E. coli, Klebsiella, Proteus, Enterococcus) 1

If the patient is asymptomatic:

  • Stop the antibiotic immediately 4
  • No further treatment or testing is needed 1, 4
  • Reassure the patient that Lactobacillus in urine is normal flora, not infection 1

Common Pitfall to Avoid

Do not treat based solely on a positive culture without considering the organism identity and clinical context. The presence of bacteria in urine does not automatically equal infection requiring treatment. 1, 4 The key distinction is whether there is a true uropathogen causing inflammation (pyuria) with symptoms, versus colonization or contamination with non-pathogenic organisms like Lactobacillus. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

The pharmacology of cephalexin.

Postgraduate medical journal, 1983

Research

Cephalexin in the therapy of infections of the urinary tract.

Postgraduate medical journal, 1983

Guideline

Treatment of Urinary Tract Infections Caused by Serratia marcescens

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.