What are the recommended tests for pleural fluid analysis in patients with pleural effusion?

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Pleural Fluid Analysis for Pleural Effusion

All pleural fluid samples should be analyzed for protein, LDH, pH, cytology, Gram stain, acid-fast bacilli (AAFB) stain, and microbiological culture, with samples sent in both sterile containers and blood culture bottles to maximize diagnostic yield. 1

Essential Initial Tests

Core Biochemical Analysis

  • Protein and LDH: Measure pleural fluid protein to differentiate transudates from exudates, using Light's criteria when protein is borderline (25-35 g/L) 1
  • pH measurement: Perform in all non-purulent effusions when infection is suspected; pH <7.2 indicates complicated parapneumonic effusion requiring drainage 1, 2
  • Glucose: Levels <60 mg/dL help identify complicated parapneumonic effusions 2

Microbiological Studies

  • Sample collection: Send 5-10 mL in aerobic and anaerobic blood culture bottles PLUS sterile containers for Gram stain, AAFB stain, and TB culture 1
  • Prioritization: When volume is limited (2-5 mL), prioritize blood culture bottles over plain containers 1

Cytological Examination

  • Volume requirements: Submit 25-50 mL for cytological analysis in suspected malignancy (strong recommendation by consensus) 1
  • Processing: Use both direct smear and cell block preparation 1
  • Limitations: Cytology detects only 60% of malignant effusions; negative results should prompt further investigation 1

Specialized Tests Based on Clinical Context

For Suspected Tuberculosis

  • Adenosine deaminase (ADA): Consider in high prevalence populations (>35-45 U/L with >50% lymphocytes suggests TB); use as exclusion test in low prevalence areas 1, 3, 2, 4
  • Interferon-gamma (IFN-γ): Can be measured with 89% sensitivity and 97% specificity 3
  • Tissue sampling: Strongly recommended as preferred diagnostic approach for all suspected TB cases (69-97% sensitivity) 1, 3

Critical caveat: ADA may be falsely elevated in empyema, parapneumonic effusions, and rheumatoid pleurisy, and may be falsely negative in HIV-positive patients 3

For Suspected Malignancy

  • Cytology sensitivity varies by tumor type: Direct biopsies should be considered for tumors with low cytological yield (especially mesothelioma) 1
  • Biomarkers: Pleural fluid biomarkers should NOT be routinely used for diagnosing secondary pleural malignancy 1

For Suspected Heart Failure

  • Serum NT-proBNP: Should be measured to support diagnosis in unilateral effusions suspected of heart failure (conditional recommendation) 1
  • Pleural fluid NT-proBNP: Not superior to serum levels and should not be ordered routinely 1

For Suspected Lupus Pleuritis

  • Pleural fluid ANA: Should be considered to support diagnosis 1

Fluid Appearance Assessment

Visual Inspection

  • Note appearance and odor: Serous, blood-tinged, frankly bloody, or purulent 1
  • Turbid/milky fluid: Centrifuge to differentiate empyema (clear supernatant) from chylothorax (turbid supernatant due to lipids) 1
  • Hemorrhagic fluid: Measure hematocrit if hemothorax suspected; pleural fluid hematocrit >50% of peripheral blood confirms hemothorax 1

Procedural Recommendations

Thoracentesis Technique

  • Image guidance: Should ALWAYS be used to reduce complications (strong recommendation by consensus) 1
  • Needle size: Use fine-bore 21G needle with 50 mL syringe 1

Diagnostic Algorithm

  1. Clinical assessment: Determine if transudate likely (heart failure, hypoalbuminemia, dialysis) 1, 5
  2. If transudate suspected: Treat underlying cause; perform thoracentesis only if atypical features or no response 5
  3. Perform thoracentesis: Send for all core tests listed above 1
  4. Classify as transudate vs exudate: Use protein levels and Light's criteria when borderline 1
  5. If exudate without diagnosis: Proceed to contrast-enhanced CT thorax and consider pleural biopsy (ultrasound/CT-guided, closed, or thoracoscopic) 1, 5

Common pitfall: Light's criteria occasionally misidentify transudates as exudates; if clinical picture suggests transudate but criteria indicate exudate, measure serum-pleural fluid albumin gradient (>1.2 g/dL confirms transudate) 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Pearls and myths in pleural fluid analysis.

Respirology (Carlton, Vic.), 2011

Guideline

Diagnostic Approach for Suspected Pleural Tuberculosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Diagnostic Protocol for Pleural Effusion

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Diagnostic principles in pleural disease.

The European respiratory journal, 1997

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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